蜂花烧烫伤膏对烧伤大鼠血管内皮细胞的保护作用研究  

Protective Effect of Fenghua BurningWound Ointment on Vascular Endothelial Cell of Severely Burned Rat

作　　者：于丹[1] 吴军凯[1] 吴修红[1] 都晓伟[1] 王宏[1] 孙慧峰[1] 

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040

出　　处：《现代生物医学进展》2014年第21期4029-4032,共4页Progress in Modern Biomedicine

基　　金：黑龙江省自然科学基金项目(D201013)

摘　　要：目的:研究蜂花烧烫伤膏对烧伤大鼠血管内皮细胞的保护作用,探讨其治疗烧烫伤的作用机制。方法:热水烧伤法建立大鼠30%TBSA深Ⅱ度烧伤模型。造模成功后随机分为对照组、烧伤延迟复苏组、京万红组与蜂花烧烫伤膏组,分别立即给予相应干预,并比较烫伤后1 h、3 h、6 h、12 h、24 h和48 h的肿瘤坏死因子-α(TNF-α)、内皮素(ET-1)、一氧化氮(NO)与ET-1/NO比值水平。结果:与对照组大鼠比较,烧伤延迟复苏组TNF-α、ET-1与ET-1/NO比值在伤后1 h即明显升高,12 h达高峰后逐渐下降;NO的含量在伤后1h即显著升高,6 h达高峰后逐渐下降;48h后仍与对照组有较大差异。与烧伤延迟复苏组比较,蜂花烧烫伤膏组各时相点的TNF-α、ET-1、NO和ET-1/NO比值均显著降低,但变化趋势基本一致。结论:蜂花烧烫伤膏通过降低TNF-α、ET-1和NO水平,优化ET-1/NO系统,起到保护烧伤大鼠血管内皮细胞的作用。Objective： To investigate the effect of Fenghua Burning Wound Ointment on vascular endothelial cell （VEC） of severely burned rat and attempt to explore its mechanisms. Methods： The 30% TBSA deep second degree burned models were established by hot water. Then the models were randomized into normal control group, burned delayed fluid resuscitation group, Jingwanhong group and Fenghua burining wound ointment group and each group was treated with drugs immediately. The levels of TNF-α, ET-1, NO and ET-1/NO were compared at 1, 3, 6, 12, 24, 48 h after rats were burned. Results： Compared with normal control group, the level of TNF-α, ET-1 and ET-1/NO increased significantly at lh, reached the peak at 12 h and then decreased slowly in bumed delayed fluid resuscitation group. The level of NO increased significantly at lh, reached the peak at 6h and then decreased slowly. Compared with burned delayed fluid resuscitation group, the levels of TNF-α, ET-1, NO and ET-1/NO decreased significantly, and the variation tendency keep in accordance in Fenghua burning wound ointment group. Conclusion： Fenghua Burning Wound Ointment can protect vascular endothelial cell of severely burned rat by reducing the levels of TNF-α, ET-1, NO and ET-1/NO.

关 键 词：烧伤 肿瘤坏死因子Α 内皮素 一氧化氮 ET-1 NO 

分 类 号：Q95-3[生物学—动物学] R644[医药卫生—外科学]