两种人胚胎干细胞体外诱导CD34^+造血前体细胞方法的比较  

Comparison of two methods to generate CD34+hematopoietic progenitor cells from human embryonic stem cells

作　　者：董晓雅[1] 潘光锦[1] 刘加军[1] 

机构地区：[1]中山大学附属第三医院血液科,广州510630

出　　处：《新医学》2014年第6期368-372,共5页Journal of New Medicine

基　　金：中国医学科学院广州健康研究院开放课题(KLRB201109)

摘　　要：目的对比小鼠骨髓基质细胞系OP9共培养和二维单层诱导方法体外诱导人胚胎干细胞(hESCs)产生CD34+造血前体细胞的效率,为后续研究提供参考。方法采用hESCs与OP9共培养方法或用二维单层诱导方法诱导hESCs产生CD34+造血前体细胞,通过流式细胞仪检测分化效率,比较两种方法的效果。结果通过hESCs/OP9共培养方法,hESCs在分化第9日出现CD34+细胞,比例达(19.7±7.9)%;通过二维单层诱导方法,hESCs在分化第6日即出现CD34+细胞,比例仅为(5.8±1.8)%,两种方法分化效率比较差异具统计学意义(P<0.05)。结论 hESCs/OP9共培养方法和二维单层诱导方法均可将hESCs诱导为造血前体细胞,相对于二维单层诱导方法,hESCs/OP9共培养方法尽管需要更长的时间,但其分化效率更高,更有利于后续研究。Objective To compare the efficiencies of two major approaches which have been developed to induce CD34 ＋ hematopoietic progenitor cells （HPCs）. They include the cocuhure of human embryonic stem cells （hESCs） with OP9 stromal cell line and two-dimensional culture system. Methods Induction of hematopoietic differentiation was conducted by coculture of hESCs with OP9 bone marrow stromal cells or through two-dimensional culture system. The efficiencies of CD34 ＋ HPCs differentiation were analyzed by flow cytometry. Results The proportion of CD34＋ HPCs developed from hESCs with OP9 cocuhure system on day 9 was about （19.7 ±7.9）%, while the proportion achieved through two-dimensional culture system on day 6 was about （5.8 ± 1.8） % （P 〈 0. 05）. Conclusion Both methods can induce HPCs from hESCs. Two-dimensional culture system require less time, but the efficiency of hESCs with OP9 coculture system is significantly higher and will be be more beneficial to the follow-up studies.

关 键 词：人胚胎干细胞 造血前体细胞 体外分化 OP9共培养 二维单层诱导 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]