人羊水特异蛋白质组学的探讨研究  

Tentative research on the human amniotic fluid proteomics

作　　者：朱斌[1] 陈芳[1] 侯常[1] 黎丽红 黄素华[1] 

机构地区：[1]南方医科大学附属何贤纪念医院产科,广州511400

出　　处：《新医学》2014年第6期390-393,共4页Journal of New Medicine

基　　金：广东省科技厅社会发展领域科技计划项目(2010B031600179)

摘　　要：目的寻找人羊水中特异性表达的蛋白质。方法羊膜腔穿刺获取3份正常孕妇的羊水，同时采集其外周肘静脉血，分离血清。提取羊水及血清中的蛋白质，用双向凝胶电泳观察人羊水和血清中蛋白质表达情况，并选取羊水中表达量较血清中高2倍以上的蛋白斑点行基质辅助激光解吸／离子化飞行时间质谱分析。结果在pH4—7、相对分子质量10-55kDa区域里，羊水中约（613±29）个蛋白点，血清中约（785±64）个蛋白点。在羊水中表达但血清中不表达的蛋白点约50个，羊水中表达量较血清高2倍以上的蛋白点约50个。结论羊水中存在特异性表达蛋白质，其在妊娠相关生理、病理学中可能起重要作用。Objective To explore the specific expression of proteomics in amniotic fluid （AF）. Methods Three samples were collected from normal AF by amniocentesis and peripheral venous blood samples were collected simultaneously from which the serum was isolated. Proteins were extracted from both samples and analyzed by two-dimensional gel electrophoresis （2-DE）. The protein blots expressed in normal AF twice as high as that in serum were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Results At pH 4-7 and within a molecular mass of 10-55 kDa, （613 ± 29） protein blots were identified in normal AF and （785± 64） in serum. Approximately 50 protein blots were expressed in normal AF rather than serum. Roughly 50 blots were expressed in normal AF twice as high as that in serum. Conclusion Specific protein expression was observed in normal AF, which probably plays a vital role in pregnancy-related physiology and pathology.

关 键 词：羊水 蛋白质组 双向凝胶电泳 基质辅助激光解吸 离子化飞行时间质谱 

分 类 号：R714.5[医药卫生—妇产科学]