致猪水肿病大肠杆菌减毒株抑制其野生菌株黏附的研究  

Research on inhibition of adhesion of edema disease-causing Escherichia coli by its attentuated variant

作　　者：陆辉[1,2] 成大荣[1] 张华[1] 朱善元[2] 夏文龙[1] 左为勇 李丽丽[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏农牧科技职业学院,江苏泰州225300

出　　处：《扬州大学学报（农业与生命科学版）》2014年第1期5-8,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省科技支撑计划(农业)项目(BE2010381);江苏省高校优势学科建设工程项目(2010-10);教育部创新团队项目(IRT0978)

摘　　要：在已构建的产志贺毒素大肠杆菌(shiga toxin-producing Escherichia coli,STEC)减毒菌株F2(O139∶F18)的基础上,进一步研究其体外对野生菌株S451521的黏附抑制作用。无菌采集断奶后1周仔猪的十二指肠,并分成若干肠段(每段2cm)试验组。将肠段分别与不同比例的减毒菌株和野生型菌株吸附后,每组取相同面积肠段并置于相同体积的LB肉汤中;充分刮取肠黏膜制成混悬液后,倍比稀释并分别涂布于LB平板上培养后细菌计数;分别挑取各组平板上的单菌落120个,通过细菌分类鉴定判定减毒菌株F2对其野生菌株的黏附抑制情况。结果表明:F2株细菌能明显抑制其野生菌对仔猪上皮细胞的黏附,抑制率在84.62%以上。该结果提示减毒菌株F2作为微生态制剂的可能性,对预防仔猪水肿病具有潜在的研究价值。Based on the toxin-gene knocked stain F2 originated from the wild type shiga toxin-producing Escherichia coli strain $451521 （O139 ： F18）, the adhesion of the wild strain $451521 inhibited by its attentuated variant were fur- ther detected in this reseach. The distal duodenum sample was aseptic cellected from the piglet after a week of weaning, and separated into several intestinal segments （each 2 cm）. Following each segment was adhered by attenuated STEC strain F2 and/or wild type strain $451521, the same size samples were cut out from each segment and put into LB broth with same volume. The intestinal mucosa were fully scraped into LB broth, and daubed on the LB plates with serial dilu- tions. Following 120 colonies were picked out from each sample and submitted to PCR examination and kanamycin-resist- ant identification, the adhensive inhibition ability of the attenuated STEC to the wild type was determinated. The result showed that the attenuated STEC strain F2 could obviously inhibit the wild-type bacteria adhesion to epithelial cells of piglets, and the inhibition rate was 84.62% at least. The data suggested that the attenuated STEC strain F2 could be used as oarl probiotics strain to prevent the edema disease.

关 键 词：仔猪水肿病 大肠杆菌 减毒菌株 黏附 抑制 

分 类 号：S852.612[农业科学—基础兽医学]