新药用资源菠萝叶的质量标准研究  

Study on Quality Control of New Medicinal Resources of Pineapple Leave

作　　者：姜敬非[1] 王伟[1] 李振国[2] 雷帆[1] 谢伟东[1] 丁怡[3] 邢东明[1] 杜力军[1] 

机构地区：[1]清华大学蛋白质科学教育部重点实验室/生命科学学院医学院药物药理实验室,北京100084 [2]河南省食品药品检验所,郑州450003 [3]清华大学生命科学学院药物发现平台,北京100084

出　　处：《世界科学技术-中医药现代化》2014年第5期972-977,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家创新药物重大专项(2012ZX09103-201-041):糖脂代谢新药BLP的研究;负责人:邢东明

摘　　要：目的:对菠萝叶质量标准进行研究,以更好的开发应用菠萝叶的药用资源。方法:利用组织切片法观察菠萝叶组织结构;利用薄层层析法以菠萝酰酯为对照品进行定性鉴别;利用高效液相色谱法对主要成分进行含量测定。结果:菠萝叶镜下可分上下表皮层,其间有气孔;薄层层析可以清晰检测到菠萝酰酯成分;高效液相色谱法可以准确的测定菠萝叶中对香豆酸和菠萝酰酯的含量,且不同产地的菠萝叶中对香豆酸和菠萝酰酯含量差异较大,其中以云南的菠萝叶含量最高,广西的菠萝叶含量最低。结论:以菠萝酰酯作定性鉴别,以对香豆酸和菠萝酰酯为指标成分进行定量检测,可以较好的控制菠萝叶药材的质量。This article was aimed to study the quality control of pineapple leaf in order to further develop the appli-cations of pineapple leave as medicinal resources. The tissue structure of pineapple leaf was observed by tissue biop-sy assay. Thin-layer chromatography （TLC） was employed on the qualitative identification of ananasate in the leave. The p-coumaric acid and ananasate were detected with high-performance liquid chromatography （HPLC） assay in the content determination. The results showed that pineapple leaf can be divided into the upper and lower leaf epidermis with stoma under microscope observation. Ananasate of the leave can be indentified clearly by TLC. HPLC methodcan accurately detect the content of p-coumaric acid and ananasate of pineapple leave. There are different contents of p-coumaric acid and ananasate of pineapple leave in different origins. In Y unnan province, the contents of p-coumaric acid and ananasate of pineapple leave are the highest; while the contents of p-coumaric acid and ananasate in Guangxi province are the lowest. It was concluded that ananasate can be used in the quality identification. The p-coumaric acid and ananasate can be used in the quantitative determination in the better control of the quality of pineapple leaf.

关 键 词：菠萝叶 质量标准 菠萝酰酯 对香豆酸 

分 类 号：R284.1[医药卫生—中药学]