补肾益髓生血法再障大鼠含药血清对大鼠造血祖细胞增殖分化及其机制的影响  

Effect of Bu-Shen Yi-Sui Sheng-Xue Method for Medicated Serum of Rats with Aplastic Anemia on Mechanism of Proliferation and Differentiation of Hematopoietic Progenitor Cells

作　　者：田晨[1] 张新雪[1] 张丰丰 赵宗江[1] 程明秀[1] 王颖超[1] 赵敬[1] 吴志奎[2] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]中国中医科学院广安门医院,北京100053

出　　处：《世界科学技术-中医药现代化》2014年第5期1076-1082,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家重点基础研究发展("973")计划(2010CB5304-06):基于"肾藏精"的脏象理论基础研究-从障碍性贫血探讨"肾生髓"理论的研究;总课题负责人:王拥军;子课题负责人:吴志奎

摘　　要：目的:探讨补肾益髓生血法再障大鼠含药血清对大鼠骨髓造血祖细胞增殖分化及其机制的影响。方法:60Co-γ射线联合环磷酰胺建立再障大鼠模型,以正常对照组、模型组、康力龙组、益髓生血组、温肾生血组和滋肾生血组复方中药灌胃大鼠,制备其含药血清培养正常大鼠骨髓细胞,培养体系中含药血清比例为20%,进行骨髓造血祖细胞集落形成单位(CFU)计数;收集集落细胞,RT-PCR检测红系GATA-1 mRNA及粒系PU.1 mRNA表达。结果:与正常对照组相比,模型组骨髓有核细胞显著减少(P<0.01),造血祖细胞红系集落形成单位(CFU-E)、红系爆式集落形成单位(BFU-E)和粒单核系造血祖细胞集落(CFU-GM)数目均明显降低(P<0.01),GATA-1、PU.1 mRNA表达显著降低(P<0.01);与模型组相比,各个治疗组大鼠骨髓有核细胞升高,造血祖细胞CFU-E、BFU-E和CFU-GM数目显著增加(P<0.01);滋肾生血组CFU-E、BFU-E明显优于益髓生血组(P<0.01);滋肾生血组CFU-GM优于益髓生血组、温肾生血组。各治疗组GATA-1、PU.1 mRNA表达明显高于模型组(P<0.01);滋肾生血组GATA-1 mRNA表达明显高于益髓生血组、温肾生血组(P<0.05);滋肾生血组PU.1 mRNA表达高于益髓生血组、温肾生血组。结论:补肾益髓生血法可能通过增加GATA-1、PU.1 mRNA表达而促进骨髓造血祖细胞增殖分化,其中滋肾生血法优于益髓生血法和温肾生血法。This study was aimed to investigate the effect of Bu-Shen Y i-Sui Sheng-Xue （BSYSSX） method on pro-liferation and differentiation mechanisms of hematopoietic progenitor cells. The rat models were established by 60Co-γrays and cyclophosphamide. Compound Chinese medicine was gavaged to rats of the normal control group, model group, stanozolol group, Yi-Sui Sheng-Xue （YSSX） group, Wen-Shen Sheng-Xue（WSSX） group and Zi-Shen Sheng-Xue （ZSSX） group. Then, serum of rat was prepared. Rat bone marrow cells were incubated with AA rats serum ac-counted for 20% and the number of hematopoietic progenitor cells colony-forming units （CFU） were counted. The level of GATA-1 and PU.1 mRNA in colony cells were detected with RT-PCR. The results showed that compared with the normal control group, the number of bone marrow cells, CFU-E, BFU-E, CFU-GM, as well as the expres-sion of GATA-1 and PU.1 mRNA in the model group decreased significantly （P〈 0.01）. Compared with the model group, the number of bone marrow cells, CFU-E, BFU-E, CFU-GM of each treatment group were significantly in-creased （P〈 0.01）. CFU-E and BFU-E of the ZSSX group were better than the YSSX group （P 〈 0.01）. CFU-GM of the ZSSX group was better than the YSSX group and the WSSX group. The expression of GATA-1 and PU.1 mR-NA in each treatment group were significantly higher than the model group （P〈 0.01）. The expression of GATA-1 mRNA in the ZSSX group was better than the YSSZ group and WSSX group （P〈 0.05）. The expression of PU.1 mR-NA in the ZSSX group was higher than the YSSX group and WSSX group. It was concluded that BSYSSX method may increase the expression of GATA-1 and PU.1 mRNA in order to promote the proliferation and differentiation of bone marrow hematopoietic progenitor cells. The ZSSX method was better than the YSSX method and WSSX method.

关 键 词：补肾益髓生血法 再障 造血祖细胞 GATA-1 PU 1 

分 类 号：R285.5[医药卫生—中药学]