星点设计-效应面法优化虎杖中白藜芦醇的提取工艺  

Optimization of Extraction Process of Resveratrol from Polygonum Cuspidatum Based on Central Composite Design and Response Surface Method

作　　者：王利洁[1] 李建利[1,2] 施明毅 罗悦[1] 陈梓贤 

机构地区：[1]成都中医药大学药学院,成都611137 [2]四川省中医药科学院中医研究所,成都610031 [3]厦门中药厂有限公司,厦门361100

出　　处：《世界科学技术-中医药现代化》2014年第5期1193-1200,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：四川省科技厅科研院所成果转化项目(2014-2-12):金利止咳颗粒新药开发;负责人:李建利

摘　　要：目的:在单因素实验结果的基础上,采用星点设计-效应面法优选白藜芦醇的提取工艺条件。方法:用有机溶剂水浴浸提法提取虎杖中白藜芦醇,以白藜芦醇含量为评价指标,采用星点设计考察溶剂浓度、提取时间和溶剂用量对提取工艺的影响,效应面法优化提取工艺条件。结果:最佳工艺条件:乙醇浓度72.0%,提取温度50℃,提取2次,每次1.1 h,溶剂用量12.7倍,白藜芦醇含量理论最大值与实测值平均偏差为0.83%。结论:该优选工艺预测性良好,可为白藜芦醇提取的工业生产提供实验依据。This study was aimed to optimize the extraction process of resveratrol by central composite design （CCD） and response surface method （RSM）, based on the result of single factor experiment. Resveratrol was extracted from Polygonum cuspidatum by water bath extraction of organic solvent. And content of resveratrol was taken as index in the investigation of solvent concentration effect, extraction time and solvent content on extraction process. The results showed that the optimum condition was 12.7 times amount of 72.0% ethanol and extract for 2 times under the temperature of 50℃, 1.1 h for each time; the average deviation between the maximum theoretical value and measured value was 0.83%. It was concluded that this extraction process was highly predictive, which provided experimental evidence for the industrial production of P. cuspidatum extraction.

关 键 词：虎杖 白藜芦醇 提取工艺 星点设计 效应面法 

分 类 号：R284.2[医药卫生—中药学]