粒细胞集落刺激因子应用后粒系细胞形态的变化分析  

Analysis of changes of myeloid cell morphology after application of G-CSF

作　　者：甄萍[1] 高志红[1] 姚新洁[1] 

机构地区：[1]衡水市哈励逊国际和平医院,河北衡水053000

出　　处：《临床血液学杂志（输血与检验）》2014年第3期481-482,共2页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:正确认识应用粒细胞集落刺激因子(G-CSF)后各阶段粒细胞的变化及形态特点,避免细胞形态学的错误判断。方法:分别采取30例应用G-CSF后的白细胞减少症患者的静脉血及骨髓制成血液涂片和骨髓涂片,常规进行瑞吉染色,以光学显微镜观察患者外周血涂片和骨髓涂片中粒系细胞的形态学变化及特点。结果:经G-CSF刺激后的患者血涂片中的粒细胞普遍存在核左移现象;骨髓中各阶段粒细胞的数量和形态都有较为明显的变化:细胞的体积增大,细胞质的含量较丰富而嗜碱性,细胞质中颗粒增多、颗粒粗大且嗜酸性。结论:应用G-CSF后,外周血和骨髓中的粒系细胞会发生一系列数量和形态上的变化,属用药反应,不同于病态造血。Objective：To correctly understand the changes and of morphological characteristics of each stage of granulocytes after using granuloeyte colony-stimulating factor （G-CSF）, and avoid the wrong judgment. Method.. Blood and bone marrow smears from venous blood and bone marrow of 30 patients with leucopenia after using G- CSF were stained by routine Raj staining, and morphological changes and characteristics of myetoid cells in the peripheral blood and bone marrow smear were observed by optical microscope. Result： After G-CSF stimulation, there were widespread nuclear left shift of granulocyte in the blood smear of patients. The number and morphology of various stages of granulocytes in bone marrow had more obvious changes., larger cell volume, higher cytoplasm content with basophilia, increased cytoplasmic granules with coarse grain and eosinophilia. Conclusion.. After the application of G-CSF, there would be a series of number and morphology changes of granulocytes in the peripheral blood and bone marrow, which was a drug reaction, different from the morbidity hematopoiesis.

关 键 词：粒细胞集落刺激因子 粒系细胞 细胞形态 病态造血 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]