二甲双胍对大鼠肾小球系膜细胞NF-κB,ICAM-1表达的影响  

Effects of metformin on expressions of nuclear factor-κB and intercellular adhesion molecule-1 in rat glomerular mesangial cells

作　　者：顾俊菲[1] 叶山东[1] 汪姗[1] 孙文佳[1] 胡圆圆[1] 

机构地区：[1]安徽医科大学附属省立医院内分泌科,安徽合肥230001

出　　处：《南京医科大学学报（自然科学版）》2014年第5期607-610,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：安徽省自然科学基金(11040606M161);安徽高校省级自然科学研究项目(KJ2011A157)

摘　　要：目的:观察二甲双胍对大鼠肾小球系膜细胞(mesangial cells,MCs)核因子-κB(nuclear factor-κB,NF-κB)、细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)表达的影响,探讨其肾脏保护机制。方法:体外培养MCs,随机分为正常糖组(NG)、高糖组(HG)及高糖+不同浓度二甲双胍组(M1:0.5 mmol/L;M2:1.0 mmol/L;M3:2.0 mmol/L)。培养48 h后收集各组细胞,采用荧光实时定量聚合酶链式反应法(real time-PCR)检测细胞NF-κB mRNA及ICAM-1 mRNA含量,蛋白质印迹法(Western blot)检测细胞NF-κBp65和ICAM-1蛋白表达水平。结果:①MCs可表达NF-κB、ICAM-1;②与NG组比较,HG组MCs NF-κB、ICAM-1mRNA和蛋白表达增强(P<0.05);③与HG组比较,M3组MCs NF-κB、ICAM-1mRNA相对表达量明显降低,差异有统计学意义(P<0.05);④与HG组比较,M1组、M2组和M3组MCs NF-κBp65、ICAM-1蛋白表达量明显降低,差异有统计学意义(P<0.01)。结论:二甲双胍可抑制肾小球系膜细胞NF-κB、ICAM-1 mRNA和蛋白表达,并具有一定的浓度依赖性,该作用可能是其肾脏保护机制之一。Objective：To observe the effects of metformin on expressions of nuclear factor-κB and intercellular adhesion molecule-1 in cultured rat glomerular mesangial cells（MCs） and explore its reno-protective mechanisms. Methods：MCs were cultured in the medium with normal glucose（group NG）, high glucose（group HG）,and different concentrations of metformin （group M1, 0.5 mmol/L;group M2, 1.0 mmol/L;group M3, 2.0 mmol/L）. After 48h exposure, MCs were collected for mRNA and protein expression of NF-κB, ICAM-1. The expression of NF-κB and ICAM-1 mRNA was analyzed by real time polymerase chain reaction. The expression of NF-κBp65 and ICAM-1 protein was visualized by western blotting . Results：①MCs expressed NF-κB and ICAM-1. ②After stimulated by high glucose, the levels of intracelluar NF-κB, ICAM-1 mRNA and protein expression were significantly increased compared with group NG（P 〈 0.05）. ③Compared with group HG, the expression of intracellular NF-κB, ICAM-1 mRNA was significantly decreased in group M3（P 〈 0.05）. ④Compared with group HG, the expression of intracellular NF-κBp65 and ICAM-1 protein was significantly decreased in group M1, M2 and M3 in a dose-dependent manner（P 〈 0.01）. Conlusion：Metformin can suppress the expression of NF-κB and ICAM-1 in glomerular mesangial cells in a concentration-dependent mode, which may partly contribute to its reno-protection.

关 键 词：二甲双胍 核因子-ΚB 细胞间黏附分子-1 

分 类 号：R349.51[医药卫生—基础医学] R587.24