国产多孔钽材料兔竖脊肌植入组织学及生物学效应  

The histology and biological effects of domestic porous tantalum implanted in rabbit erector spinae

作　　者：甘洪全[1] 刘鑫[2] 赵济华[3] 耿丽鑫 王茜[5] 王志强[1] 李琪佳[4] 

机构地区：[1]河北联合大学附属医院骨科,河北唐山063000 [2]唐山开滦总医院检验科,河北唐山063000 [3]河北联合大学临床医学院,河北唐山063000 [4]河北联合大学临床医学院实验中心,河北唐山063000 [5]河北联合大学临床医学院基础医学院解剖教研室,河北唐山063000

出　　处：《南京医科大学学报（自然科学版）》2014年第5期611-616,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家科技部科技支撑项目(2012BAE06B03)

摘　　要：目的：观察国产多孔钽材料的三维孔隙结构以及兔竖脊肌植入后组织形态学特点,对其组织相容性进行评价,为临床骨缺损修复重建提供实验依据。方法：扫描电镜观察国产多孔钽结构;成年新西兰大白兔24只,建立多孔钽兔竖脊肌植入动物模型。将2枚直径1.5 cm。厚0.2 cm圆型多孔钽植入脊柱一侧竖脊肌内（实验组）,对侧植入同等数量及体积的多孔钛（对照组）。于术后2。4。8。12 周处死行硬组织切片观察肌肉周围纤维包膜及新生血管形成,并计算小血管生成数量;扫描电镜观察肌肉组织在两种材料内的生长状态。结果：扫描电镜观察多孔钽具有三维立体多孔结构,孔隙直径在400－600 μm;肌肉及纤维组织早期在多孔钽表面及孔隙内生长;晚期胶原纤维延展,相互连接并充满孔隙。硬组织切片显示：随时间的延长多孔钽-肌肉界面形成薄而致密的血管化包膜。孔隙内充满肌肉。纤维组织及小血管。结论：国产多孔钽材料具有良好的组织相容性,其三维结构可使肌肉组织在表面及孔隙内生长及延伸,有可能成为骨缺损修复材料。Objective：To observe the three-dimensional （3D） structure and morphologic characteristics of domestic porous tantalum implanted in rabbit erector spinae and evaluate its histocompatibility,and to provide experimental evidence for repair and reconstruction of bone defects. Methods：The structure of the domestic porous tantalum was observed by scanning electron microscope （SEM）. A total of 24 adult New Zealand rabbits were used to establish porous tantalum model. Two 1.5 cm × 1.5 cm × 0.2 cm porous tantalum materials were implanted in one side of rabbit erector spinae （the experimental group）. Two 1.5 cm × 1.5 cm × 0.2 cm porous titanium materials （the control group） were implanted into the opposite side in the same rabbits. The rabbits were sacrificed successively at 2,4,8 and 12 weeks post implantation. Slices of hard tissue specimen were examined to observe fibrous capsule morphology and vascular formation. Muscle and fibrous tissue morphology around the material were observed by SEM. Results：The SEM showed the 3D structure of porous tantalum and pore diameter ranged from 400 to 600 μm. The muscle and fibrous tissue ingrowth was found in the surface and pores of porous tantalum in the early stage. In the late stage,collagen fibers and the pores of porous tantalum were filled with muscle tissue and connected to each other. Hard tissue slices observed that the loose and thick vascularized fibrous capsule formed in the porous tantalum-muscle interface at the beginning and became thin and compact in the end. The muscles,small vessels and fibrous tissues were found in the pores of materials. Conclusion：The Chinese porous tantalum has good histocompatibility,and its 3D porous structure is advantageous to ingrowth and extension on the surface and in the pores for the muscle tissue. The Chinese porous tantalum may has the potential to repair bone defect.

关 键 词：多孔钽 组织相容性 形态学 血管化包膜 

分 类 号：R318.08[医药卫生—生物医学工程]