不同应激模式下血清中重要应激激素的变化规律  

Effect of different military stress loads and modes on level of stress hormones in rats

作　　者：王新兴[1] 杲修杰[1,2] 弓景波[1] 梅竹松[1] 刘伟丽[1] 马婧[1] 赵云[1] 钱令嘉[1] 

机构地区：[1]军事医学科学院基础医学研究所,全军军事认知与心理卫生研究中心,北京100850 [2]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《军事医学》2014年第4期277-280,共4页Military Medical Sciences

基　　金：国家自然科学基金资助项目(81000584;81372124)

摘　　要：目的：系统观察不同军事应激模式和军事应激负荷下大鼠血清中重要应激相关激素的变化规律。方法以改良水环境多平台睡眠剥夺法（ MMPM）建立睡眠剥夺模型；以自制束缚仓建立大鼠束缚应激模型；以控制动作、使笼潮湿、使笼倾斜、通宵照明、禁食禁水、空瓶刺激、成组饲养等7种温和应激方式建立大鼠慢性不可预计温和应激（ CUMS）模型；分别检测上述3种应激模式下不同应激负荷大鼠血清中皮质醇、儿茶酚胺类和5-HT水平的变化规律。结果睡眠剥夺5d和7d大鼠血清GC含量显著高于对照组，而血清儿茶酚胺类含量则在睡眠剥夺1d显著升高；CUMS干预4周后大鼠血浆GC显著高于对照组，血清5-HT水平则显著低于对照组，血清儿茶酚胺类含量在2～8周均显著高于对照组；束缚应激大鼠1周后儿茶酚胺类显著升高，3～4周后则恢复到正常水平，而GC含量则在2～4周显著高于对照组。结论在不同的应激模式下大鼠血清GC水平随应激负荷增加而逐渐升高，可以作为评价不同应激模式下应激负荷的激素指标；儿茶酚胺类和5-HT则不具备作为类似指标的特征。Objective To systematially observe the different patterns and leves of military stress on modified multitle-platform method the level of stress-related hormones in rats .Methods A sleep deprivation model was established by water environment modified multiple-platform method（MMPM）.A restraint stress model was established by self-made bondage cage.Chronic unpredictable mild stress （ CUMS） model was established by action control , cage damp, cage tilt, night lightening, water and food fasting , empty bottles stimulation and group feeding methods .Cortisol, catecholamines and 5-HT levels were detected to observe the different military stress loads and modes on the level of stress hormones .Results The level of serum cortisol increased significantly （ P 〈0.05 ） after 5 days of sleep deprivation .The level of serum catecholamines increased significantly （P〈0.05） after 1 day of sleep deprivation.The cortisol concentration increased and the level of 5-HT decreased in serum after 4 weeks CUMS.The level of catecholamines increased significantly （P〈0.05） after 2-8 weeks of CUMS, increased significantly （P〈0.05） after 1 week of restraint stress , and returned to normal after 3-4 weeks of restraint stress .The level of serum cortisol increased significantly after 3 -4 weeks of restraint stress . Conclusion Cortisol levels gradually increased with the level of stress in different military stress modes , which can serve as an index to evaluate the level of different stress modes .

关 键 词：军事应激负荷 军事应激模式 激素 

分 类 号：R828.4[医药卫生—临床医学]