高温下极低频电磁场暴露对果蝇hsp22和hsp26基因影响的研究  

Co-effects of extremely low frequency electromagnetic field (ELF-EMF) and temperature on hsp22 and hsp26 expression in Drosophila melanogaster

作　　者：张晶[1] 张紫燕[1] 杨传俊[1] 蔡澎[1] 

机构地区：[1]中国科学院城市环境研究所城市环境与健康重点实验室,福建厦门361021

出　　处：《军事医学》2014年第5期321-326,共6页Military Medical Sciences

基　　金：中国科学院装备研制资助项目(Y2I0041A20;Y0G5221A20);中国科学院城市环境研究所资助项目(09L6321A90)

摘　　要：目的探讨在高温(35℃)条件下,极低频电磁场(extremely low frequency electromagnetic fields,ELF-EMF)对雌雄黑腹果蝇hsp22和hsp26基因表达水平的影响。方法在25、27.5、30、32.5、35℃下,1日龄雌、雄黑腹果蝇在50 Hz、3 mT ELF-EMF暴露2 h,测定其死亡率、运动能力和hsp22、hsp26表达水平;在25和35℃下,1日龄雌、雄黑腹果蝇在50 Hz、3 mT ELF-EMF暴露2、4、8、24 h,测定其hsp22、hsp26表达水平。结果在25、27.5、30、32.5、35℃下暴露2 h,温度变化对果蝇的死亡率、运动能力和hsp22、hsp26表达水平有极显著影响(P<0.01),ELF-EMF对hsp22、hsp26表达水平无显著影响(P>0.05);在25和35℃下分别暴露2、4、8、24 h,性别、温度、ELF-EMF暴露和暴露时间对hsp22、hsp26的表达水平有极显著影响,其中,温度是主要的影响因素,且若干因素间有交互作用。结论ELF-EMF可影响果蝇hsp22、hsp26的表达,且ELF-EMF可能具有降低因高温环境所致负面影响的作用,提高果蝇对高温环境的耐受能力,缓解高温环境对果蝇的损伤效应。Objective To study the combined effect of extremely low frequency electromagnetic fields （ ELF-EMF ） exposure （50 Hz and 3 mT） and high temperature exposure on hsp22 and hsp26 expression in Drosophila melanogaster. Methods Under the conditions of 50 Hz and 3 mT ELF-EMF,the male and female D.melanogaster was separately exposed for 2 h at 25℃, 27.5℃, 30℃, 32.5℃,and 35℃, respectively.The death rate, exercise capacity and the hsp22 and hsp26 expression levels of D.melanogaster were detected after exposure;or at 25℃and 35℃at 2 h, 4 h, 8 h, and 24 h, respectively.The hsp22 and hsp26 expression levels of D.melanogaster were tested after exposure .Results After exposure for 2 h at 25℃, 27.5℃, 30℃, 32.5℃, and 35℃, temperature had a significant effect （P〈0.01） on the death rate, exercise capacity and the hsp22 and hsp26 expression levels of D.melanogaster, but ELF-EMF had no significant effect （P〉0.05）.After exposure for 2 h at 25℃, 27.5℃, 30℃, 32.5℃, and 35℃, the hsp22 and hsp26 expression levels of D.melanogaster were significantly impacted（P〈0.01） by gender, temperature, ELF-EMF and exposure time, and tem-perature was the most important factor .Gender, temperature, ELF-EMF and exposure time had some interaction with the results.Conclusion ELF-EMF can influence the expression levels of hsp22 and hsp26.ELF-EMF exposure contributes to heat tolerance in D.melanogaster through accelerated expression of hsp22 and hsp26.

关 键 词：极低频电磁场 高温 黑腹果蝇 hsp22 hsp26 

分 类 号：Q786[生物学—分子生物学]