法舒地尔对经皮冠状动脉介入治疗患者的内皮保护作用  

Protective effects of fasudil on endothelium in patients after percutaneous coronary intervention

作　　者：徐萌萌[1] 谭丽娟[1] 潘娜娜[1] 宇仁超[1] 

机构地区：[1]青岛大学医学院附属医院心内科,山东青岛266555

出　　处：《岭南心血管病杂志》2014年第3期310-313,共4页South China Journal of Cardiovascular Diseases

基　　金：山东省自然科学基金自助项目(项目编号:ZR2013HM016)

摘　　要：目的观察法舒地尔对择期行经皮冠状动脉介入治疗(percutaneous coronary intervention,PCI)的患者血清中细胞因子ROCK I、肿瘤坏死因子-α、一氧化氮(nitric oxide,NO)表达的影响。方法选择因不稳定型心绞痛择期行PCI治疗的患者为试验组,随机(按电脑随机数字表法)分为两组:常规药物治疗组(A组)和常规药物加法舒地尔治疗组(B组)。选择经冠状动脉造影(coronary angiography,CAG)证实为非冠状动脉粥样硬化性心脏病(冠心病)的患者为对照组。采用酶联免疫吸附法(ELISA)测定血清中ROCK I、肿瘤坏死因子-α的表达,硝酸还原酶法测定NO的表达。结果 PCI治疗后,与对照组相比,试验组血清中ROCK I、肿瘤坏死因子-α浓度明显升高,A组较B组更高;NO浓度降低,A组较B组更低,差异有统计学意义(P均<0.05)。结论 PCI治疗能损伤冠状动脉内皮细胞,法舒地尔能有效降低血清中炎性细胞因子水平,升高NO浓度,具有保护冠状动脉内皮细胞功能的作用。Objectives To explore the effects of fasudil on cytokine ROCK I, tumor necrosis factor-α（TNF-α）, nitric oxide （NO） expressions in patients performed percutaneous coronary intervention （PCI）. Methods Patients with unstable angina performed PCI were selected as experimental group. They were randomly divided into two subgroups： conventional drug treatment group （subgroup A） and conventional drug and fasudil treatment group （ subgroup B ）. Patients without coronary heart disease confirmed by coronary angiography were selected as control group. Expressions of ROCK I and TNF-ot were measured by enzyme-linked immunosorbent assay （ELISA）. Expression of NO was measured by nitrate reduetase assay. Results Compared with control group, serum concentrations of ROCK I and TNF-α were significantly higher after PCI in experimental group, and those of subgroup A were higher than those of subgroup B; While serum concentration of NO in experimental group was lower, that of subgroup A was lower than that of subgroup B （all P〈0.05）. Conclusions Coronary artery endothelial cells can be damaged by PCI. Fasudil can effectively reduce the serum concentrations of inflammatory cytokines and increase the NO concentrations to protect coronary artery endothelial cells.

关 键 词：血管成形术 经腔 经皮冠状动脉 法舒地尔 RHO激酶 肿瘤坏死因子-Α 一氧化氮 

分 类 号：R541.4[医药卫生—心血管疾病]