麋鹿角对D-半乳糖诱导小鼠衰老模型抗衰老作用  

Effect of Elaphuri Davidiani Cornu on the Aging Model Induced by D-galactose in Mice

作　　者：李锋涛[1,2] 段金廒[1] 钱大玮[1] 郭建明[1] 蒋情 彭蕴茹[3] 丁玉华 任义军 

机构地区：[1]南京中医药大学、江苏省中药资源产业化过程协同创新中心/中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023 [2]江苏农牧科技职业学院,江苏泰州225300 [3]江苏省中医药研究院,江苏南京210028 [4]江苏省大丰麋鹿国家级自然保护区,江苏大丰224136

出　　处：《南京中医药大学学报》2014年第3期235-238,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81274017);江苏省“六大人才高峰”项目(第七期);江苏省博士后科研项目(1002021C)

摘　　要：目的研究麋鹿角抗衰老作用。方法采用D-半乳糖诱导的小鼠亚急性衰老模型,以麋鹿角粉、水提取物和水提药渣灌胃给药,检测血浆中白细胞介素-2(IL-2)、肿瘤坏死因子?α(TNF?α)水平,肝、肾和脑中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量,脑中单胺氧化酶(MAO)活性,小鼠背部皮肤羟脯氨酸(Hyp)含量,以及胸腺和脾脏指数。结果麋鹿角粉和水提取物均能显著提高衰老小鼠肝脏、肾脏以及脑组织内过低的SOD活性,提高肾脏和脑组织内过低的GSH-PX活性;同时降低肝脏和脑组织内过高的MDA水平,抑制脑组织中过高的MAO活性,显著升高过低的脾脏和胸腺指数;显著升高小鼠背部皮肤中过低的Hyp含量。水提取药渣也能显著提高肾脏中SOD和GSH-PX活性,抑制脑组织内MDA和MAO水平,升高背部皮肤中Hyp含量和胸腺指数。结论麋鹿角可通过提高机体抗氧化酶系活性,抑制脑组织MAO活性,提高免疫功能,延缓皮肤衰老而发挥抗衰老作用。本研究可为麋鹿角药用资源的合理利用及其产业化提供科学依据。OBJECTIVE To study the anti-aging effect of Elaphuri Davidiani Cornu（EDC）.METHODS An aging model induced by D-galactose in mice was established;the powder of EDC,the water extract of EDC and the resulting residue were tested;the content of interleukin-2（IL-2）and tumor necrosis factorα（TNF-α）in plasma,the activities of superoxide dismutase（SOD）and glutathione peroxidase（GSH-Px）,and the malondialdehyde（MDA）content in liver,kidney and brain,the activity of MAO in brain,the hydroxyproline content in the back skin of mice were evaluated.In addition,the viscera index of spleen and thymus were also measured.RESULTS Compared to the model group,both of the powder of EDC and the water extract of EDC could significantly increase the SOD activity in liver,kidney and brain,the GSH-Px activity in kidney and brain,the viscera index of spleen and thymus,and the hydroxyproline content in the back skin of mice.And they could also decrease the MDA content in liver and brain,and the MAO in brain.The resulting residue could significantly increase the SOD and GSH-Px activities in kidney,the hydroxyproline content in the back skin,and the viscera index of thymus.In addition,it could also decrease the MDA and MAO in brain.CONCLUSION The mechanism of anti-aging of EDC might be related to the elevation activities of antioxidant enzymes,suppression activities of MAO in brian,and enhancement of immune function,to slow down skin aging.The present study may also provid a rational basis for comprehensive utilization of the resources of EDC and its resources industrialization.

关 键 词：麋鹿角 抗衰老 D-半乳糖衰老模型 

分 类 号：R285.5[医药卫生—中药学]