梓白皮、桑白皮和楸树皮对H_2O_2致损的L-O2肝细胞的保护作用  

Protective Effects of Bark of Chinese Catalpa,Bark of Catalpa Bungei and White Mulberry Root-bark on Liver Cell Line L-O2 Injuries Induced by H_2O_2

作　　者：吴军[1] 王明艳[2] 周春祥[2] 

机构地区：[1]南京中医药大学翰林学院,江苏泰州225300 [2]南京中医药大学基础医学院,江苏南京210023

出　　处：《南京中医药大学学报》2014年第3期254-258,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家"十一五"科技支撑计划(2006BAI11B08);江苏省研究生科研创新计划项目(2010)

摘　　要：目的研究梓白皮、楸树皮和桑白皮对体外H2O2致损肝细胞的保护作用及其机制。方法采用H2O2(100μmol/L)体外诱导L-O2肝细胞损伤,CCK-8法检测细胞活性,检测细胞上清中天门冬氨酸转换酶(AST)、丙氨酸氨基转换酶(ALT),超氧化物歧化酶(SOD)和丙二醛(MDA)水平,ELISA法检测细胞中TNF-α和IL-1水平,流式细胞术检测细胞周期。结果 H2O2可以改变L-O2细胞的形态结构,抑制其活性;促进细胞代偿性增殖;提高细胞AST、ALT和TNF-α、IL-1的水平;降低细胞SOD活性。而梓白皮、楸树皮和桑白皮可拮抗H2O2对细胞的损伤。结论梓白皮、楸树皮和桑白皮对H2O2致损的LO2肝细胞有保护作用。OBJECTIVE To investigate the protective effect and mechanism of Bark of Chinese catalpa（Xinbaipi）,bark of catalpa bungei（Jiushupi）and white mulberry root-bark（Sangbaipi）on damaged liver cell line L-O2 induced by H2O2 in vitro. METHODS Liver cell line L-O2 were incubated and then damaged by H2O2（100μmol/L）in vitro.The cell activity was detected by CCK-8 method.The levels of AST,ALT and MDA in cell supernatant were detected according to the kit.Levels of TNF-aand IL-1were detected by ELISA,and cell cycle was analyzed by flow cytometry.RESULTS H2O2 could change morphological structure of L-O2 cells,inhibit their activities,increase levels of AST,ALT,TNF-α and IL-1and inhibit activation of SOD in cells.However,bark of Chinese catalpa,bark of catalpa bungei and white mulberry root-bark could inhibit these injuries.CONCLUSION Bark of Chinese catalpa,bark of catalpa bungei and white mulberry root-bark can protect L-O2 cells from in vitro injuries induced by H2O2.

关 键 词：梓白皮 楸树皮 桑白皮 L-O2细胞 过氧化氢 保护作用 

分 类 号：R285.5[医药卫生—中药学]