大腹皮的HPLC指纹图谱研究  

Study on HPLC Fingerprints of Arecae Pericarpium

作　　者：汤丽娟[1] 吴皓[1,2,3] 郁红礼[1,2,3] 

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药炮制重点实验室,江苏南京210023 [3]国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210023

出　　处：《南京中医药大学学报》2014年第3期276-279,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：<中华人民共和国药典>一部药品标准课题(779)

摘　　要：目的建立大腹皮药材的指纹图谱。方法 Waters SCX色谱柱(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(浓氨试液调节pH为4.3)(80∶20),等度洗脱,柱温30℃,检测波长为215nm,流速为1.0mL/min。结果采用相似度评价法,对所收集的12批大腹皮药材样品进行系统比较和归类,确定了6个共有峰,指认了其中4个,分别为槟榔次碱、去甲槟榔次碱、槟榔碱、去甲槟榔碱的色谱峰,并采用聚类分析、主成分分析方法,将不同产地的药材样品分为3大类。结论本法重复性好,简便可靠,可作为大腹皮的质量控制和评价依据。OBJECTIVE To establish HPLC fingerprint of Arecae Pericarpiurn ~rom different areas and provide a reliable method for its scientific evalution and quality control. METHODS The determination was performed on Waters SCX（250 mm ×4.6mm,5μm）eolumn with mobile phase consisted of acetonitril-0.1% phosphoric acid solution（adjusted to pH=4.3 with ammonium hydroxide） at the flow rate of 1.0 mL/min. The detection wavelength was set at 215 nm, and the column tempera ture was maintained at 30 ℃. RESULTS There were 6 common peaks in the fingerprints and 3 categories of 12 samples. Arecoline hydrobromide, arecaidine hydrochloride, guvacoline hydrobromide and guvacine hydrochloride were identified. The similarity of 12 batches of Arecae Pericarpiurn was more than 0.92. CONCLUSION The method is rapid and efficient, and can be used for the quality evaluation and control of Arecae Pericarpium.

关 键 词：大腹皮 HPLC 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]