外源基因转化烟草悬浮细胞方法的改进  

Method Improvement in Transformation of Foreign Gene into Tobacco Cell Suspension

作　　者：郑帮[1] 陈燕[1] 周树敏[1] 张卫[1] 

机构地区：[1]上海大学生命科学学院,上海200444

出　　处：《河南师范大学学报（自然科学版）》2014年第3期115-118,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：上海市教委浦江人才计划(08PJ1405500);国家自然科学基金面上项目(30870225)

摘　　要：烟草悬浮培养细胞作为一种重要的实验材料已有较多的研究,将外源基因通过克隆进行载体构建,转化入悬浮培养的烟草细胞并进行表达,在带有相应筛选抗性的抗生素固体培养基中进行培养,再将愈伤组织转入液体培养基中进行培养,得到转入外源基因的液体悬浮培养烟草细胞.我们通过对此法作一些改进,使其便于操作、省时,适合在一般实验室条件下进行操作,且烟草悬浮培养细胞传代培养快,取材方便的特性对实验的开展也有很大的促进.Suspension cell of tobacco is an important experimental materials for many researches. The foreign gene expression vector was constructed by gene cloning and transformed into tobacco cell suspension. After screening on solid medium containing antibiotics, the callus were transferred into liquid medium in order to get the transgenic cell suspension of tobacco. We improved the method with simply operation and timesaving, in order to make the method applied to general laboratory conditions. Furthermore, tobacco cell suspension with quikly subculture and easy collection, will promote the research progress.

关 键 词：外源基因 转化 烟草悬浮培养细胞 方法 

分 类 号：Q754[生物学—分子生物学]