玉米大斑病田间接种鉴定方法探索  

Exploration of Northern Corn Leaf Blight Injection in Field

作　　者：刘欣芳[1] 朱迎春 李明[1] 马骏[1] 王延波[1] 姜敏[1] 冯桂荣 

机构地区：[1]辽宁省农业科学院玉米研究所,辽宁沈阳110161 [2]辽宁省种子管理局,辽宁沈阳110034 [3]沈阳市康平县张强镇区域性农业技术推广站,辽宁沈阳110500

出　　处：《辽宁农业科学》2014年第3期46-48,共3页Liaoning Agricultural Sciences

基　　金：国家自然基金面上项目小额资助(31371627);农业部转基因生物新品种培育重大专项(2013ZX08003003-008)

摘　　要：以玉米大斑病鉴别寄主A619近等基因系为供试材料,以玉米大斑病1号、2号、3号、N号生理小种组成的混合小种为接种病菌,通过单叶病斑面积的发展速率评价鉴别寄主接种后的发病情况,分析了完全隔离法、部分隔离法与不设置隔离田间接种鉴定效果。结果表明:与不设置隔离法相比,完全隔离法和部分隔离法均能成功接种病菌,完全隔离法发病程度高于部分隔离法,但操作相对比较繁琐,适宜按生理小种鉴定的小规模接种实验。部分隔离法选择适宜接种天气条件,适合大规模群体鉴定。A619 hosts were chosen as testing materials,Race 1,2,3,N of northern corn leaf blight（NCLB）were mixed for injection,Rate of lesion development was chosen for measuring the hosts severity after injection.We compared the result of entirely isolation system（EIS）,partially isolation system（PIS） and non-isolation method（NIM）.The results showed that EIS and PIS can inject successfully comparing with NIM.EIS incident is severer than PIS with more complex than PIS and which is suitable for accurate injection by separate races.PIS is suitable for population injection when the weather is appropriate.

关 键 词：玉米大斑病 鉴别寄主 完全隔离法 部分隔离法 病斑发展速率 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]