灵芝粗多糖对二乙基亚硝胺诱导的小鼠肝脏组织形态及原癌基因和抑癌基因的影响  

Effects of Ganoderma lucidum Crude Polysaccharide on Liver Tissue Morphology,and Expression of Proto-oncogene and Tumor Suppressor Gene mRNA,in Diethylnitrosamine-treated Mice

作　　者：冯嫣[1] 王家东[2] 冯翠萍[1] 常明昌[1,3] 程红艳 孟俊龙[1] 

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801 [2]信阳农林学院生物技术系,河南信阳464000 [3]山西省食用菌工程技术研究中心,山西太谷030801 [4]山西农业大学资源与环境学院,山西太谷030801

出　　处：《食用菌学报》2014年第1期42-46,F0003,共6页Acta Edulis Fungi

基　　金：山西省自然科学基金项目(编号:2010011043-1);山西省科技基础条件平台建设项目(编号:2010091017);山西省科技攻关项目(编号:20130311017-3)的部分研究内容

摘　　要：采用苏木精-伊红染色法(hematoxylin-eosin staining)和实时荧光定量PCR(real-time PCR,RTPCR)法,研究灵芝(Ganoderma lucidum)粗多糖对二乙基亚硝胺(diethylnitrosamine,DEN)诱导的小鼠肝脏组织形态以及原癌基因H-ras和myc和抑癌基因P53、Rb1mRNA的影响,结果表明:灵芝粗多糖对DEN引起的小鼠肝脏损伤具有缓解作用,并可以抑制原癌基因的表达、提高抑癌基因的表达。Sixty healthy,four week-old,mice were randomly divided into six groups （female∶male=1∶1 ）as follows;normal control,diethylnitrosamine （DEN）model group,Ganoderma lucidum polysaccharide （GP） group,and low （DEN ＋ 50 mg/kg·d）,median （DEN＋100 mg/kg·d）and high （DEN＋200 mg/kg·d）GP dose groups.After 45 days,liver tissue morphology was observed using hematoxylin-eosin staining,and proto-oncogene （ras and H-myc）and tumor suppressor gene （p53 and Rb1）mRNA expression levels were determined using fluorescent quantitative real-time polymerase chain reaction （RT-PCR ）. Our data demonstrated that GP had the protective function on liver cells of DEN-treated mice,and inhibited to a limited extent the occurrence or development of cancer by regulating the expression of proto-oncogenes and tumor suppressor genes.

关 键 词：灵芝粗多糖 肝组织形态 原癌基因 抑癌基因 

分 类 号：R285[医药卫生—中药学]