高灵敏特异性红霉素印迹传感器用于血样中红霉素的测定  

Application of Highly Sensitive and Specific Erythromycin-imprinted Sensors for Determination of Erythromycin in Blood Samples

作　　者：宋彬[1] 周雨笋[1] 荆涛[1] 周廷廷[1] 刘敏[2] 郝巧玲[1] 周宜开[1] 梅素容[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院环境医学研究所、教育部环境与健康重点实验室,武汉430030 [2]华中科技大学化学与化工学院,武汉430074

出　　处：《华中科技大学学报（医学版）》2014年第3期249-253,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：新世纪优秀人才计划(No.NCET-11-0178);教育部博士点基金(No.20120142110012);武汉市科技攻关计划资助项目(No.2013020501010181)

摘　　要：目的构建一种高灵敏特异性分子印迹电化学传感器用于血样中红霉素（erythromycin，ERY）的检测。方法采用沉淀聚合法，以ERY为模板分子，优化模板分子与功能单体之间的摩尔比和交联剂的用量，制备一种分子印迹聚合物（molecularly imprinted polymers，MIPs），并利用其特异识别能力与乙炔黑（acetylene black，AB）高催化活性相结合，构建一种分子印迹电化学传感器，用于血样中ERY的检测。结果该传感器对ERY具有良好的选择性以及较高的灵敏度，印迹因子为3．6。检测线性范围为1．0×10^-8～5．0×10^-6mol／L（r^2=0．9978），检出限为4．3×10mol／L。该法用于人血清中ERY的测定，加标回收率为82．0％～119．8％。结论建立了一种高灵敏特异性分子印迹电化学传感器，能用于血样中ERY的快速检测，为指导ERY的使用和保护人体健康提供有力的技术支持。Objective To construct a highly sensitive and specific molecularly imprinted electrochemical sensor for detecting erythromycin （ERY） in blood samples. Methods After optimizing the molar ratio of the template molecule （ERY） to functional monomer and the amount of cross linker, molecularly imprinted polymers （MIPs） was prepared by precipitation polymerization. Then,a molecularly imprinted electrochemical sensor was constructed by combining MIPs of specific recognition ability with acetylene black （AB） of highly catalytic activity to detect ERY in blood samples. Results This sensor was highly selective and sensitive for ERY, with the imprinting factor of 3.6. The linear range of the sensor was 1.0× 10^-8 - 5.0 ×10^-6 mol/L（r^2= 0. 997 8）,with a limit of detection （LOD） of 4.3×10^-9 mol/I.. The recovery of the spiked ERY in blood samples was found to be from 82.0% to 119.8%. Conclusion A highly sensitive and specific molecularly imprinted electrochemical sensor was constructed. The sensor can be used for rapidly detecting ERY in blood samples and is expected to provide technical support for guidance of the use of ERY and protection of human health.

关 键 词：红霉素 分子印迹聚合物 乙炔黑 碳糊传感器 血样 

分 类 号：R446.39[医药卫生—诊断学]