Angiopep-2修饰纳米颗粒穿越血脑屏障的能力  

Capacity of Angiopep-2 Modified Nanoparticles across the Blood Brain Barrier

作　　者：熊志勇[1] 王旋[1] 张志平[2] 谭松巍[2] 赵洪洋[1] 姜晓兵[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经外科,武汉430022 [2]华中科技大学同济医学院药学院,武汉430030

出　　处：《华中科技大学学报（医学版）》2014年第3期304-306,310,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.81272778)

摘　　要：目的研究Angiopep-2修饰纳米颗粒穿越血脑屏障的能力。方法分别制备载香豆素-6和罗丹明B标记的纳米颗粒(NP)和Angiopep-2修饰的纳米颗粒(ANP),将其与脑毛细血管内皮细胞(BCECs)共培养,以荧光显微镜和激光扫描共聚焦显微镜观察BCECs对Angiopep-2修饰纳米粒的摄取能力。体内小鼠尾静脉注射纳米粒,以荧光显微镜观察并比较2种纳米粒在脑组织中的分布情况。结果在荧光显微镜和激光扫描共聚焦显微镜下,BCECs摄取ANP的荧光强度明显强于摄取NP的荧光强度;小鼠体内脑组织摄取的ANP显著多于NP。结论经Angiopep-2修饰的纳米颗粒具有比普通纳米颗粒更强的穿越血脑屏障的能力。Objective To study the capacity of angiopep-2 modified nanoparticles penetrating the blood brain barrier. Methods The nano particles （NPs） marked by coumarin-6 and rbodamine B or by angiopep-2 were prepared and then cocultured with brain capillary endothelial cells （BCECs）. The uptake of NPs by BCECs was observed by fluorescence microscopy and laser scanning confocal microscopy. The distribution of the two kinds of NPs in brain tissues was observed by fluorescence microscopy after injection of NPs into mice via the tail vein. Results The fluorescence microscopy and laser scanning confocal microscopy showed that the BCECs that absorbed angiopep-2 gave much brighter fluorescence than those absorbing coumarin-6 and rhodamine B. The uptake of angiopep-2-modified NPs was greater in brain tissue in rats. Conclusion Angiopep-2-modified NPs possess higher capacity of penetrating the blood brain barrier than common NPs.

关 键 词：angiopep-2 纳米颗粒 脑靶向 血脑屏障 

分 类 号：R318.08[医药卫生—生物医学工程]