眼镜蛇毒对成年大鼠脊神经节BDNF和c-jun表达的影响  

Effects of Naja naja atra venom on BDNF and c-jun expression in spinal ganglion of adult rats

作　　者：周荣海[1] 丁见[1] 熊克仁[1] 

机构地区：[1]皖南医学院人体解剖学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2014年第3期193-195,共3页Journal of Wannan Medical College

摘　　要：目的:探讨眼镜蛇毒对成年大鼠脊神经节脑源性神经营养因子(BDNF)和c-jun表达的影响。方法:大鼠24只随机分为正常对照组、生理盐水组和眼镜蛇毒组,每组8只。用免疫组织化学的方法,观察在各组大鼠脊神经节BDNF和c-jun的表达。结果:大鼠脊神经节BDNF和c-jun眼镜蛇毒组细胞平均灰度值低于正常对照组和生理盐水组(P<0.01),且阳性神经元眼镜蛇毒组多于正常对照组和生理盐水组(P<0.01)。结论:眼镜蛇毒能上调脊神经节BDNF和c-jun的表达,这种表达增加可能与脊神经节损伤的代偿性修复有关。Objective： To investigate the effects of Naja naja atra（ N. naja,Chinese cobra） venom on the expression of brain-derived neurotrophic factor（ BDNF） and c-jun in spinal ganglion of adult rats. Methods： Twenty-four adults rats were randomized into control group,saline administration group and N. naja venom treatment group（ n = 8 for each）. Immunohistochemistry technique was used to detect the expression of BDNF and c-jun in spinal ganglion in each group of rats. Results： The mean gray value of BDNF and c-jun in spinal ganglion was significantly lower in N. najia treatment group than that of saline administration group and control group（ P〈0. 01）,and the number of BDNF and c-jun positive cells were also greater than the latter two group（ P〈0. 01）. Conclusion： N. naja venom can significantly increase the BDNF and c-jun expression in spinal ganglion of rats,and this mechanism may be associated with compensatory healing of spinal ganglion injury.

关 键 词：眼镜蛇毒 BDNF C-JUN 脊神经节 大鼠 

分 类 号：R965[医药卫生—药理学]