马鞍山地区汉族人群15个STR基因座遗传多态性调查  

Investigation on the genetic polymorphism of 15 STR loci in Han population in Ma'anshan area

作　　者：陈蓉华[1] 侯杰[1] 晏斌[1] 周豫军[1] 

机构地区：[1]马鞍山市公安局刑警支队刑科所,安徽马鞍山243000

出　　处：《皖南医学院学报》2014年第3期263-265,共3页Journal of Wannan Medical College

摘　　要：目的:调查292名马鞍山地区汉族无关个体15个STR基因座的等位基因类型及其频率。方法:用硅珠法提取DNA,采用AmpFISTR Identifiler荧光标记复合扩增系统进行复合扩增;扩增产物用ABI3130xl型遗传分析仪检测,得到STR分型结果后统计15个基因座的基因频率。结果:15个STR基因座,累计个人识别率达0.999 999 999 999 999 985,累计非父排除率为0.999 998 96。结论:该系统在马鞍山地区汉族人群中具有高度多态性,适用于法医学亲权鉴定、个体识别以及DNA数据库的建立。Objective： To investigate the alleles and their frequencies of 15 short tandem repeates（ STR） loci from292 unrelated individuals of Han nationality living in Ma＇anshan area. Methods： DNA was extracted with silica particles,amplified by PCR with AmpF1STR Identifiler kit,and analyzed with ABI3130XL Genetic Analyzer. The repeated sequences were statistically summed up for the 15 STRs after genotyping. Results： In the 15 STR loci of Ma＇anshan Han population,the total discrimination power（ TDP） was 0. 999 999 999 999 999 985 and 0. 999 998 96 for the combined probabilities of paternity exclusion. Conclusion： The 15 STR loci has higher genetic polymorphism in the Han population in Ma＇anshan area,and may be applied to paternity testing,individual identification and DNA database reference.

关 键 词：法医物证学 STR基因座 遗传多态性 汉族 

分 类 号：D919[医药卫生—法医学]