二元线性回归分析在旋光法测定复方甘露醇注射液含量中的应用  

Application of Dualistic Linearity Regression Analysis in Content Determination of Compound mannitol Injection by Polarimetry

作　　者：赖坤平[1] 陈日檬 温坚[1] 韦国麟[1] 林永春[1] 

机构地区：[1]湛江中心人民医院,湛江524037 [2]湛江市药品检验所

出　　处：《数理医药学杂志》2014年第3期256-258,共3页Journal of Mathematical Medicine

摘　　要：目的:介绍二元线性回归分析在旋光法测定复方甘露醇注射液含量的两种应用方法。方法:先直接测定复方甘露醇标准溶液旋光度α1,然后以钼酸铵或硼砂为增旋剂,与适量的复方甘露醇标准溶液作用后测定旋光度αm或αp,所得数据经二元线性回归分析,求回归方程,然后根据回归方程和样品的旋光度计算出复方甘露醇注射液的含量。结果:不管是钼酸铵还是硼砂为增旋剂,旋光度与甘露醇、葡萄糖的浓度均呈良好的线性关系,钼酸铵为增旋剂回归方程的复相关系数为:r1=0.9998,rm=0.9992;硼砂为增旋剂回归方程的复相关系数为:r1=0.9998,rp=0.9989。甘露醇和葡萄糖回收率均很好。结论:上述两种方法均操作快捷、简便实用,所需的仪器普通,结果准确可靠,重复性好,表明二元线性回归分析与旋光法结合测定复方甘露醇注射液的含量有很好的应用价值,可用于成品和半成品的质控。Objective：To introduce two ways from dualistic linearity regression analysis to determine the content of injections that compound mannitol by polarimetry.Methods：Determine the optical rotationα1from Standard solution of compound mannitol injections,take ammonium molybdate or borax as spiral agents,mixed with Standard solution of mannitol injections compound recipe,Determine the optical rotationαmorαp,all data analyzed based on dualistic linearity regression analysis,through the regression equation and the optical rotation determined,figure out the content of compound mannitol injection.Results：Both ammonium molybdate and borax as spiral agents,the results show a linear correlation between the mannitol and gluconic.ammonium molybdate as spiral agents demonstrated good linearity with r1=0.9998,rm =0.9992,borax as spiral agents demonstrated good linearity with r1=0.9998,rm=0.9989.The recovery of them shows well.Conclusion：Two kinds of methods we mentioned are quick,simple and practical operation,all instrument we needed are common,the results show accurate,reliable,in good repeatability,all demonstrate dualistic linearity regression analysis in combination with polarimetry to determine the content of compound mannitol injection with good application,that can be used for quality control in finished products and semi-finished products.

关 键 词：二元线性回归 旋光法 含量测定 甘露醇 葡萄糖 

分 类 号：R927.2[医药卫生—药学]