5-(4-氯苯基偶氮)-8-苯基磺酰氨基喹啉与铜(Ⅱ)的荧光反应及分析应用研究  

Study on the Fluorescence Reaction of Copper(Ⅱ) with 5-(4-Chlorophenylazo)-8-Benzenesultonamidoquinoline and Its Analytical Applications

作　　者：叶艳青[1] 曹秋娥[2] 徐其亨[2] 

机构地区：[1]云南民族学院化学系,昆明市莲花池650031 [2]云南大学化学系,昆明市650091

出　　处：《光谱实验室》2001年第2期219-222,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：研究了一种新试剂 :5- ( 4 -氯苯基偶氮 ) - 8-苯基磺酰氨基喹啉 ( CPBSQ)在聚乙烯醇存在下与铜 ( )的荧光反应。结果表明 ,在 p H=7.5的硼砂 -磷酸二氢钠缓冲溶液中 ,铜 ( )与 CPBSQ形成 1∶ 2的稳定配合物 ,在 λex/ λem=32 7nm/ 368nm产生强荧光。依此建立一种高灵敏和选择性的荧光分析方法 ,该法能检测0 .10— 50 μg/ L Cu( ) ,其检出限为 1.0 6ng/ L。用此法测定了废水、人发和食品中铜的含量 ,结果令人满意。A fluorescence reaction of copper(Ⅱ) with 5-(4-chlorophenylazo)-8-benzenesultona-midoquinoline (CPBSQ) was firstly studied.CPBSQ could react with Cu(Ⅱ) in the medium of Na_2B_4O_7-NaH_2PO_4 buffer solution (pH=7.5) to form a 1∶2 complex,which showed an intensive fluorescence at λ_ex/λ_em=327nm/368nm.Based on this fluorescence reaction,a sensitive and selective fluorescent method for the determination of Cu(Ⅱ) was developed.The linearity range of this method is over 0.10—50μg/L with a detection limit of 1.06ng/L.The method has been successfully used to the determination of copper in waste water,hair and food samples.

关 键 词：铜(Ⅱ) 荧光光度法 5-(4-氯苯基偶氮)-8-苯基磺酰氨基喹啉 废水 人发 食品 分析 

分 类 号：O614.121[理学—无机化学] O657.32[理学—化学]