人血清对生殖支原体LAMPs诱导THP-1细胞表达TNF-α的影响  

Effect of Human Serum on Lipid-associated Membrane Proteins of Mycoplasma Genitalium Induced TNF-α Production in THP-1 Cells

作　　者：何军[1,2] 游晓星[2] 伍绍坚 田巍[1] 吴移谋[2] 

机构地区：[1]南华大学附属南华医院检验科,湖南衡阳42100 [2]南华大学病原生物学研究所

出　　处：《中南医学科学杂志》2014年第3期226-228,245,共4页Medical Science Journal of Central South China

基　　金：国家自然科学基金课题(31100137);湖南省自然科学基金课题(14JJ7044);湖南省卫生厅科研计划课题(B2011-058)

摘　　要：目的探讨人血清对生殖支原体(Mg)脂质相关膜蛋白(LAMPs)诱导TNF-α表达的影响。方法提取对数期Mg的LAMPs,分别加入脂蛋白脂肪酶,蛋白酶K及人血清预处理后,刺激THP-1细胞,ELISA法测定其TNF-α水平。结果 Mg LAMPs为一混合蛋白,其活性成分为脂质成分,能激活THP-1细胞表达大量TNF-α,且与LAMPs浓度呈明显的剂量依赖性。人血清能上调LAMPs诱导的TNF-α水平,且与LAMPs浓度呈明显的剂量依赖性。结论人血清上调Mg LAMPs诱导THP-1细胞表达TNF-α。Objective To investigate the effects of human serum on TNF-α expression activated by lipid-associated membrane proteins （ LAMPs） of Mycoplasma genitalium（ Mg） . Methods LAMPs were extracted and pretreated with lipo-protein lipase or protease K or serum human. THP-1 cells were stimulated and the expression of TNF-α was detected by ELISA. Results LAMPs are mixed proteins and the active ingredients are lipid moiety. The secretion of TNF-αwas media-ted in LAMPs induced THP-1 cells and was significantly increased by LAMPs in a dose-dependent manner. TNF-αlevels were significantly up-regulated in LAMPs activated THP-1 cells by Human serum and was significantly increased by LAMPs in a dose-dependent manner. Conclusions The human serum raised TNF-αlevels by Mg LAMPs induced THP-1 cells.

关 键 词：生殖支原体 脂质相关膜蛋白 肿瘤坏死因子 人血清 

分 类 号：R375[医药卫生—病原生物学]