丁香属12种植物花粉保存方法研究  

Storage methods of 12 species of lilac( Syringa L.) pollen

作　　者：刘芊[1,2] 徐瑾[1,2] 石印[1,2] 孟昕[3] 刘燕[1,2] 

机构地区：[1]北京林业大学园林学院,北京100083 [2]国家花卉工程技术研究中心,北京100083 [3]北京植物园,北京100093

出　　处：《浙江农业学报》2014年第3期615-620,共6页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金项目(31370693)

摘　　要：丁香属植物是重要的园林观赏植物。试验测定了丁香属12种植物鲜花粉在室温(25℃)、冰箱(4℃)、液氮罐(-196℃)中保存不同时间的花粉活力。研究结果表明,在25℃保存条件下,11种花粉可保存至7 d,其中有5种保存30 d后仍有活力;在4℃条件下,12种丁香花粉均可保存至30 d,其中红丁香和紫丁香保存180 d后仍有生活力,但萌发率显著低于新鲜花粉萌发率,分别为2.19%和1.5%;在-196℃条件下,有11种花粉能够保存至180 d,且基本与新鲜花粉萌发率无显著差异。因此,超低温保存是较长期保存丁香属植物花粉活力的有效途径。Lilac（ Syringa L.） are important garden plants with abundant species in China. The research on the pollen preservation of lilac is of significant meanings for germplasm conservation and hybridization breeding. The germination of 12 species of fresh lilac pollen preserved in room temperature（ 25℃）,refrigerator（ 4℃） and liquid nitrogen（ LN2,-196℃） were determinate respectively after different storage time. The results demonstrated that the11 species of lilac pollen preserved at 25℃ can be preserved for 7 d,and 5 species of them can survive after 30 d.Whereas,12 species of lilac pollen preserved at 4℃ can be preserved for as long as 30 d,but only S. villosaand and S. oblata were alive after 180 d,and the germination rate（ 2. 19% and 1. 50%,respectively） was significantly lower than the fresh pollen. In contrast,under-196℃,11 species of lilac pollen can be preserved for 180 d,and there were no significant difference from the fresh pollen in germination rate.

关 键 词：丁香 花粉 超低温保存 

分 类 号：S685.26[农业科学—观赏园艺]