不同处理方法对3种大丽花病毒的脱毒效果比较  

Comparison of different detoxification methods on three Dahlia virus

作　　者：杨婷[1] 崔志刚[2] 陈段芬[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071000 [2]河北农业大学国土资源学院,河北保定071000

出　　处：《浙江农业学报》2014年第3期621-625,共5页Acta Agriculturae Zhejiangensis

基　　金：河北省林业局资助项目(1118411)

摘　　要：为了进一步完善大丽花脱毒技术,获得优质大丽花脱毒苗,以感染大丽花花叶病毒(DMV)、黄瓜花叶病毒(CMV-D2)和烟草花叶病毒(TMV)的大丽花为试材,采用茎尖结合热处理、化学处理方法对大丽花脱毒效果进行了探讨。各培养单株经酶联免疫法(ELISA)检测,结果表明,茎尖结合热处理能有效脱除DMV和CMV-D2,脱毒率分别达到74.7%和66.9%,但不能有效脱除TMV,对3种病毒均脱除的比率为10.6%;茎尖结合化学处理能有效脱除DMV,CMV-D2和TMV,脱除率分别为83.1%,80.5%和77.1%,对3种病毒的脱除率为24.3%。In order to improve the detoxification technology of Dahlia pinnata,acquire high-quality virus-free seedlings,Dahlia pinnata infected with dahlia mosaic virus（ DMV）,cucumber mosaic virus（ CMV-D2） and tobacco mosaic virus（ TMV） were used as experimental material,the effects of heat treatment,chemical treatment methods combined with stem tip culture on detoxification were studied in this paper. The results of enzyme-linked immunoassay（ ELISA） showed that stem tip in combination with heat treatment could effectively remove the DMV and CMV-D2,and virus-free rate was 74. 7% and 66. 9% respectively,but it could not remove TMV effectively,while the removal rate of three kinds of viruses was 10. 6%. Stem tip method combined with chemical treatment could effectively remove the DMV,CMV-D2 and TMV,and virus-free rate was 83. 1%,80. 5% and 77. 1% respectively,while virus-free rate of all the three was 24. 3%.

关 键 词：大丽花 茎尖 热处理 化学处理 脱毒 

分 类 号：S682.261[农业科学—观赏园艺]