浙江省水稻立枯丝核菌5.8S rDNA-ITS区间多样性  

Diversity research on Rhizoctonia solani isolated from Zhejiang Province using sequence analysis of 5.8S rDNA-ITS interval

作　　者：邱海萍[1,2] 毛雪琴[2] 姜华[2] 王艳丽[2] 孙国昌[2] 

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310058 [2]浙江省植物有害生物防控重点实验室--省部共建国家重实验室培育基地,浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

出　　处：《浙江农业学报》2014年第3期702-707,共6页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31071644);农业部转基因专项(2011ZX08009-003-001);浙江省公益性重大项目(2011C12901-1;2011C12901-2;2011C12022);浙江省农科院国际合作项目(2009)

摘　　要：对浙江省82个水稻立枯丝核菌菌株及13个标准融合群菌株5.8S rDNA-ITS(ITS)区间测序和分析,结果表明,ITS在标准融合群菌株间的差异远大于AG1-IA融合群的水稻菌株间的差异,进化分析表明,ITS的差异可以有效甄别不同标准融合群菌株,但对AG1-IA融合群的水稻菌株区分能力非常有限。单倍型分析表明,这82个水稻菌株可以分为10个单倍型。田间菌株的单倍型多样性(Hd)为0.208 7,核苷酸多样性为0.000 33。其中单倍型H1为优势单倍型,占全部菌株的87.8%,而其他9个单倍型则各只有1株菌株。中性检验结果表明Tajima's D测验值为-2.260 12(P<0.01),Fu和Li's D测验值为-5.208 27(P<0.02),暗示浙江省水稻纹枯菌菌株显著偏离中性假说,在历史上可能出现过种群扩张,并存在很强的选择作用。Eighty-two R. solani strains and 13 standard anastomosis group strains were used in the sequence analysis of 5. 8S rDNA-ITS（ ITS） interval. The differences in sequences of ITS interval among anastomosis group strains were much larger than that among the AG1-IA strains. Phylogenetic analysis showed that the differences in sequences of ITS interval could effectively distinguish strains from different anastomosis groups. But when the strains belonging to the same anastomosis group were concerned,the usage of the differences in sequences of ITS interval was quite limited. Haplotype analysis showed that 82 rice strains could be divided into 10 haplotypes. Haplotype diversity（ Hd） of these field isolates was 0.2087,and the total nucleotide diversity was 0.00033. H1 was the dominant haplotype,accounting for 87. 8% of all strains,while each of the other nine haplotypes only contained one strain.Neutral test results showed that Tajima＇s D test value was-2. 26012（ P〈0. 01） and Fu and Li＇s D test value was-5. 20827（ P〈0. 02）,implying that rice sheath blight strains might significantly deviate from neutral equilibriumhypothesis that the R. solani population may be under expansion in the history under strong selection pressure.

关 键 词：立枯丝核菌 水稻 转录间隔区 序列测定 单倍型 

分 类 号：Q78[生物学—分子生物学]