颈盲蝽取食对薇甘菊叶片防御性酶活性的影响  

Effects of Pachypeltis sp. feeding on defense enzyme activities in Mikania micrantha leaves

作　　者：季梅[1] 泽桑梓[1,2] 赵宁[3] 杨斌[3] 

机构地区：[1]云南省林业科学院,云南省森林植物培育与开发利用重点实验室,云南昆明650201 [2]云南林业职业技术学院,云南昆明650224 [3]西南林业大学,云南昆明650224

出　　处：《浙江农业学报》2014年第3期748-751,共4页Acta Agriculturae Zhejiangensis

基　　金：国家林业公益性行业科研专项项目(201204518);云南省社会发展科技计划项目(2012CH001);云南省应用基础研究面上项目(2010CD131)

摘　　要：为探明颈盲蝽Pachypeltis sp.取食对薇甘菊叶片内主要防御酶活性的影响,以薇甘菊为材料,室内条件下测定颈盲蝽取食12,24,48,96 h后薇甘菊叶片中过氧化物酶(POD)、多酚氧化酶(PPO)及苯丙氨酸解氨酶(PAL)的活性。结果表明,颈盲蝽取食后薇甘菊叶片中的POD和PAL的活性下降,PPO活性提高。取食48 h后,处理组叶片中POD和PAL活性分别下降了51.91%和75.00%,而相应的对照组叶片则分别下降了32.34%和49.86%;处理组和对照组叶片PPO活性分别上升了371.73%和121.13%。To study the effects of feeding by Pachypeltis sp. on the defense enzymes in Mikania micrantha,activities of three enzymes,peroxidase（ POD）,polyphenol oxidase（ PPO） and phenylalanine aminolyase（ PAL）,were evaluated in M. micrantha leaves at time points of 12h,24h,48h and 96h post-feeding under laboratory conditions.The results showed that activities of POD and PAL decreased while PPO increased. At 48 h after feeding,POD and PAL activities of the treatment group decreased by 51. 91% and 75. 00% respectively,while that of the control group decreased by 32. 34% and 49. 86%. PPO activity of the treatment group increased by 371. 73%,while that of the control group increased by 121. 13%.

关 键 词：薇甘菊 颈盲蝽 取食 防御酶活性 

分 类 号：S451[农业科学—植物保护]