H_2O_2-Co^(2+)产生的羟自由基的溴邻苯三酚红氧化法检测  

Assay of Hydroxyl Radical Produced in Co^(2+)-H_2O_2 Reaction System by Spectrophotometry with Bromopyrogallol Red

作　　者：吴南[1] 任凤莲[1] 吴心传[2] 

机构地区：[1]中南工业大学化学化工学院,湖南长沙410083 [2]湖南教育学院化学系,湖南长沙410012

出　　处：《分析测试学报》2001年第2期52-54,共3页Journal of Instrumental Analysis

基　　金：国家自然科学基金重点项目! (29773012)

摘　　要：提出了Co2 +-H2O2-溴邻苯三酚红分析新体系并用于羟自由基的测定。该法用Co2 +与H2O2 反应 ,类似Fenton试剂产生羟自由基 (·OH) ,并加入溴邻苯三酚红显色剂 ,使溴邻苯三酚红的颜色发生变化 ,采用紫外-可见分光光度计测定其ΔA值的变化 ,可间接测定羟自由基的产生量。通过测定条件的研究 ,得出最佳实验条件。结果表明 ,该法稳定性好 ,操作简便 ,测定快速 ,可作为一种简便的筛选抗氧化剂的方法。A new system Co2+-H2O2-bromopyrogallol red for investigating hydroxyl radical(·OH) is reported. Resembling Fenton agent, the system produces hydroxyl radical by reaction between Co2+ and H2O2. After hydroxyl radical was produced, bromopyrogallol red is added. The amount of hydroxyl radical produced in the system can be indirectly assayed through absorbance change at a wavelength of 560 nm(ΔA560) by means of UV-Vis spectrophotometer. The optimal experimental conditions were evaluated. The method proved to be rapid, simple and stable. It can be used to select antioxidants.

关 键 词：羟基自由基 溴邻苯三酚红 过氧化氢 硫酸钴 氧化 测定 分光光度法 

分 类 号：O621.146[理学—有机化学] O657.32[理学—化学]