铕-芦氟沙星的氧化产物-EDTA配合体系的敏化荧光  

Fluorescence System of Eu^(3+)-Oxidation Productof Rufloxacin-EDTA and Its Application

作　　者：刘建群[1] 赵慧春[2] 

机构地区：[1]萍乡高等专科学校化工系,江西萍乡337000 [2]北京师范大学化学系,北京100875

出　　处：《分析测试学报》2001年第2期77-79,共3页Journal of Instrumental Analysis

摘　　要：提出了测定人体尿液中芦氟沙星的Eu3 +敏化荧光法 ,研究了芦氟沙星在HAc介质中被H2O2 氧化的反应机理。在近中性HAc -NaAc缓冲溶液中芦氟沙星的氧化产物与Eu3 +、EDTA形成三元络合物 ,产生Eu3 +的特征荧光 (λem 617nm、λex 352nm) ,其荧光强度与芦氟沙星的浓度成线性关系。尿液标准曲线线性范围5.0×10-8～2.5×10-6 mol/L ,检出限1.5×10-8 mol/L。方法简单、快速、灵敏。Rufloxacin can be oxidized by H2O2 in HAc solution at 100 ℃, and its fluorescence is lost. A ternary complex of the oxidation product with EDTA and Eu3+ can be formed in the HAc-NaAc buffer solution. The complex has characteristic fluorescence of Eu3+ at an emission wavelength of 617 nm(λex=352 nm). The linear relationship between fluorescence intensity and concentration of rufloxacin in the range of 5.0×10-8～2.5×10-6 mol/L was found. A method for the determination of rufloxacin in human urine was establised by Eu3+ sensitized fluorescence analysis in view of the above. Under the selected optimum conditions, the detection limit of the method is 1.5×10-8 mol/L. The method is simple, rapid and sensitive. The mechanism of the oxidation reaction of rufloxacin in HAc solution was also discussed according to the changes of UV, fluorescence and infrared spectra.

关 键 词：芦氟沙星 氧化反应 铕 敏化荧光 尿液 抗菌药 药物分析 氧化产物 EDTA 配合物 

分 类 号：R978.19[医药卫生—药品] R969.1[医药卫生—药学]