微流控芯片快速鉴定多重细菌  

Rapid Identification of Multiple Bacteria on a Microfluidic Chip

作　　者：文小霞[1] 徐邦牢[1,2] 王伟鑫[1] 梁广铁[1,2] 陈斌[1,2] 杨银梅[1,2] 刘大渔[1,2] 

机构地区：[1]广州医科大学附属广州市第一人民医院检验医学研究室,广州510180 [2]广东省临床分子医学及分子诊断重点实验室,广州510180

出　　处：《分析化学》2014年第6期791-798,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.81371649;81171418;81201163);国家重大科学仪器设备开发专项(No.2011YQ0301240503);广州市科技计划项目(Nos.2010U1-E00681;2012J4100030);广州市医药卫生科技项目(Nos.20121A021002;20121A011035)资助~~

摘　　要：建立了一种用于多重细菌鉴定的微流控芯片分析方法。在芯片上实现细菌进样、培养和鉴定,结合培养池阵列的空间分辨力以及菌种特异性显色培养基的颜色分辨力,可以实现多重细菌检测。实验选用4种泌尿系统感染常见病原菌作为模拟测试对象,结果显示,这种芯片方法在15 h内可完成细菌鉴定,检测限可达101cfu/mL。临床样本测试结果显示,芯片方法可以实现4种细菌同步鉴定,检测结果与传统方法的一致性达到96.3%。这种微流控细菌鉴定方法简便快速,有望发展成为细菌检测的有力工具。We developed a microfluidic device to integrate sample introduction,bacteria culturing and results reading. The identification of multiple bacteria was achieved by combining the spatial resolution of the arrayed bacteria culture chambers and the color resolution benefited from the bacteria specific chromogenic media. A set of 4 common pathogenic bacteria responsible for urinary tract infection were used as a model to test the microfluidic assay. Our results showed that the bacteria identification assay can be completed in 15 h,with a limit of detection（ LOD） of bacteria density down to 10 cfu / mL. Clinical sample testing using the microchip approach showed a coincidence rate of 96. 3% as compared with the conventional method. The developed microfluidic approach is simple and rapid,thus hold the potential to serve as a powerful tool for detection of multiple bacteria.

关 键 词：微流控芯片 细菌鉴定 多重显色反应 快速检测 

分 类 号：Q93-3[生物学—微生物学]