竹材木素酚对牛血清蛋白吸附和解吸特点的研究  

Research on Sorption and Desorption Features of Bamboo Lignophenols to Bovine Serum Albumin(BSA)

作　　者：任浩[1] 船岡正光 

机构地区：[1]南京林业大学轻工科学与工程学院,江苏南京210037 [2]日本三重大学生物资源学研究科

出　　处：《纤维素科学与技术》2014年第2期1-7,共7页Journal of Cellulose Science and Technology

基　　金：江苏省自然科学基金青年基金(BK2012420)

摘　　要：通过相分离技术,在竹材木质素的苄基引入不同的酚类化合物(对甲酚、邻苯二酚、间苯二酚、连苯三酚)得到不同结构性质的木质素衍生物——木质素酚(木素对甲酚、木素邻苯二酚、木素间苯二酚、木素连苯三酚),通过GPC分析木质素酚的分子量,同时,用考马司亮蓝法研究不同结构的木质素酚和牛血清蛋白之间的吸附和解吸关系。结果表明,四种木质素酚随羟基增加对蛋白质吸附能力增强,竹材木质素酚表现出与木材木质素酚相当的对蛋白质吸附能力。此外,由于竹材木质素末端含有羧基,当溶液pH变化时,特别是木质素多酚在解吸过程中更容易受电荷影响。Through phase separation technique, Bamboo lignin was introduced withp-cresol, catechol, resorcinol, pyrogallol on benzyl position and separated from Bamboo lignocellulosics as four kinds of lignopheols （ligno-p-cresols, lignocatechol, lignoresorcinol, lignopyrogallol）. The molecular weights were characterized through GPC analysis. The protein adsorption abilities of all kinds lignophenols were compared using Bradford test method. Bovine Serum Albumin （BSA） was used as testing protein, they reacted with lignophenols in buffer solutions. Results showed that the adsorption ability of lignophenols increased with their hydroxyl and carboxyl groups content. Additionally, lignopolyphenols showed good enough desorption ability for cyclic utilization in acidic or alkaline conditions.

关 键 词：竹材 木质素酚 牛血清蛋白 吸附 解吸 

分 类 号：TQ93[化学工程] TS79[轻工技术与工程—制浆造纸工程]