大鼠脑出血后IL-1β和CD163/HO-1信号通路表达及其与脑水肿的相关性  

The relationship of the CD163/HO-1 pathway and IL-1β and edema in tissue around experimental cerebral hemorrhagic rats

作　　者：刘宝华[1] 白光辉[1] 邬伟[1] 林海燕[1] 商萍[1] 王志翊[1] 王小同[1] 

机构地区：[1]温州医科大学附属第二医院,温州325027

出　　处：《浙江创伤外科》2014年第3期348-350,共3页Zhejiang Journal of Traumatic Surgery

基　　金：浙江省医药卫生平台骨干人才计划项目(2013RCA036);温州市科技计划项目(Y20130040)

摘　　要：目的探讨脑出血后CD163/HO-1信号通路与IL-1β表达的动态变化,分析其与脑水肿的关系。方法 SD大鼠90只,随机分为正常对照组、假手术组、模型组、分别在造模后24小时、72小时、7天处死动物,免疫组化法检测HO-1、CD163、IL-1β的表达,并测定脑组织含水量。结果 ICH大鼠模型组24h后灶周可见IL-1β、HO-1、CD163阳性细胞增多,IL-1β阳性细胞数脑出血组与假手术组相比增多(P<0.01),假手术组IL-1β阳性细胞数多于正常对照组(P<0.01)。HO-1阳性细胞数脑出血组、假手术组均高于正常对照组(P<0.01),脑出血组高于假手术组,在72小时明显增多(P<0.01),CD163假手术组在72小时高于正常对照组(P<0.01),各时间点脑出血组明显高于假手术组及正常对照组(P<0.01)。脑组织含水量72小时最高,与HO-1、CD163正相关(P<0.05)。结论 CD163、HO-1与ICH后炎性因子IL-1β的表达相关,CD163/HO-1信号通路与ICH后脑水肿有关。Objective To investigate the relationship between the CD163/HO-1 pathway, IL-1βand edema in perihematoma tissue of exper-imental cerebral hemorrhagic rats. Methods The rat model of experimental ICH was established by injection of arterial blood into the caudate nu-cleus, the expression of HO-1, CD163 and IL-1β were detected by using immunohistochemistry at different time. Brain water content was measured. Results The expression of HO-1,CD163 and IL-1β appeared at 24 hour after ICH, peaked at 72 hour, then decreased gradually, there was a posi-tive correlation between HO-1,CD163 and IL-1β（P〈0.05）, and the brain water content（P〈0.05）. Conclusions The CD163/HO-1 pathway played an important role in inflammatory reactions in perihematoma tissue around experimental ICH rats, and was correlated with encephaledema of the ICH rats.

关 键 词：脑出血 CD163/HO-1通路 脑水肿 白介素-1 

分 类 号：R651.1[医药卫生—外科学]