中药茯苓抗肿瘤有效组分研究  

Anti-Tumor Effective Fractions of Fuling

作　　者：王晓菲[1] 刘春琰 窦德强[1] 

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《辽宁中医杂志》2014年第6期1240-1244,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：973课题(2013CB531803);2013辽宁省高等学校创新团队(LT2013020)

摘　　要：目的:茯苓及其化学拆分组分抗胃癌细胞SGC-7901和乳腺癌细胞Bcap-37增殖的有效部位的研究。方法:MTT法和血清药理学方法测定茯苓石油醚、乙酸乙酯、多糖、大孔吸附树脂水洗物(WEF)和大孔吸附树脂醇洗物(AEF)五个不同组分对胃癌细胞SGC-7901和乳腺癌细胞Bcap-37增殖的抑制率。结果:茯苓多糖对乳腺癌细胞的半数抑制率IC50为29.29μg/mL,乙酸乙酯为57.84μg/mL,血清药理学实验结果显示茯苓多糖和茯苓乙酸乙酯组分吸光度值与对照组比较有显著性差别(P<0.01),与阳性组比较无显著性差异(P>0.05)。茯苓多糖对胃癌细胞的IC50为25.38μg/mL,乙酸乙酯组分对胃癌细胞的IC50为56.27μg/mL,血清药理学实验结果显示茯苓多糖和茯苓乙酸乙酯组分吸光度值与对照组比较有显著性差别(P<0.01),与阳性组比较无显著性差异(P>0.05)。结论:茯苓抗胃癌和乳腺癌的活性组分一致为茯苓多糖和乙酸乙酯组分,并存在一定的时间和量效关系,为进一步研究茯苓抗肿瘤作用提供科学依据。Objective： To study the anti-proliferation effect on SGC-7901 cell and Bcap-37 cell of Fuling extracts. Methods： The proliferating inhibition of the five Fuling extracts including petroleum ether fraction,EtOAc fraction,polysaccharides,alcohol eluated fraction（ AEF） and water eluated fraction（ WEF） from macroporous absorption resin against SGC-7901 cell and Bcap-37 cell was assessed by MTT assays and seropharmacology method. Results： The IC50of polysaccharides on Bcap-37 cell was 29. 29 μg /mL,that of EtOAc was 57. 84 μg /mL. The OD values of Polysaccharides and EtOAc fraction were decreased compared with those of the control group（ P 〈 0. 01）,and there was no difference between the fractions and positive group（ P 〉0.05） in the experiment of seropharmacology. The IC50on SGC-7901 of polysaccharides and EtOAc fraction was respectively 25.38 μg /mL and 56. 27 μg /mL. The OD values of Polysaccharides and EtOAc fraction were decreased compared with those of the control group（ P 〈 0. 01）,and there was no difference between the fractions and positive group（ P 〉 0. 05） in the experiment of seropharmacology. Conclusion： The effective fractions of Fuling against Bcap-37 cell and SGC-7901 cell are polysaccharides and EtOAc fraction in a time and dose dependent manner,which provides evidence for the further study on anticancer activity of Fuling.

关 键 词：茯苓有效部位 MTT 血清药理学 5-FU 胃癌细胞SGC-7901 乳腺癌细胞Bcap-37 

分 类 号：R284.1[医药卫生—中药学]