黄芪卫矛合剂调节糖尿病肾病大鼠肾组织细胞因子平衡的研究  

Study of Huangqi Weimao Mixture Regulating Unbalance Cytokine in Renal Tissue of Diabetic Nephropathy Rat

作　　者：张丽芬[1] 吕仁和[2] 李伟[1] 刘兰英[1] 刘文毅[1] 郭力[1] 王蕊[1] 姚燕[1] 

机构地区：[1]北京中医药大学第三附属医院肾内科,北京100029 [2]北京中医药大学附属东直门医院肾病内分泌科,北京100700

出　　处：《辽宁中医杂志》2014年第6期1269-1272,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金项目(30500671)

摘　　要：目的:观察黄芪卫矛合剂对糖尿病肾病模型大鼠肾组织VEGF、TSP-1和NO、ET-1的影响,探讨黄芪卫矛合剂治疗糖尿病肾病的作用机制。方法:采用单侧肾切除并链脲佐菌素尾静脉注射建立大鼠糖尿病肾病模型,造模成功后,大鼠随机分为假手术组、糖尿病肾病组、糖尿病肾病+黄芪卫矛合剂组和糖尿病肾病+氯沙坦钾组,共观察12周。用免疫组织化学染色法观察大鼠肾小球内血管内皮细胞生长因子(VEGF)和血小板反应蛋白1(TSP-1)的表达,硝酸还原化学比色法测定肾组织NO的含量,放射免疫法测定肾组织ET-1含量。结果:假手术组肾小球内VEGF强表达,TSP-1偶见有表达;糖尿病肾病大鼠肾小球内VEGF明显减弱,而TSP-1表达明显增多(与假手术组比较P<0.01);各用药组VEGF表达较模型组有明显增多,TSP-1表达均明显减少,与模型组比较有显著性或非常显著性差异(P<0.05,P<0.01)。两用药组之间两两比较没有显著性差异(P>0.05)。糖尿病肾病模型组大鼠肾组织内NO含量较假手术组明显降低(P<0.01),ET-1含量较假手术组明显升高(P<0.05),黄芪卫矛合剂组大鼠肾组织内NO含量明显增加,ET-1含量明显降低,与模型组比较有显著性差异(P<0.05);氯沙坦钾组肾组织NO和ET-1含量与模型组比较无显著性差异(P>0.05)。结论:黄芪卫矛合剂可增加肾小球内VEGF表达,降低TSP-1表达,提高肾组织中NO含量,降低ET-1含量,调节与肾小球内皮细胞密切相关细胞因子VEGF、TSP-1和NO、ET-1的平衡,从而保护肾组织微小血管内皮细胞,促进毛细血管和小血管再生和重建。是黄芪卫矛合剂防治糖尿病肾病的重要作用机制之一。同时证明了黄芪与卫矛配伍科学性和有效性,也是中医气血关系理论的进一步验证。Objective：To study the effect of Huangqi Weimao mixture on glomerular VEGF,TSP - 1, NO and ET - 1 of diabetic nephropathy rat. To explore the treatment mechanism of Huangqi Weimao mixture on diabetic nephropathy. Methods ： The diabetic nephropathy model in rats was established with unilateral kidney and streptozotocin intravenously. After the success of modeling, the rats were randomly divided into sham operation group, diabetic nephropatby group, diabetic ＋ Huangqi Weimao mixture group and diabetic nephropathy ＋ losartan potassium group. The experiment continued for 12 weeks. We observed glomerular vascular endothelial cell growth factor and thrombospondin - 1 expression by immunohistochemical staining. We also observed NO and ET - l contents in renal tissue. Results ： The VEGF had strong expression in glomerular of sham operation rats while TSP - 1 was oc- casionally expressed. VEGF expression was decreased obviously in model rats, and the expression of TSP - 1 increased significant- ly in model rats （P 〈0.01 ,compared with sham operation group）. VEGF expression was increased and TSP- 1 expression was significantly reduced in two treatment groups and there was significant difference compared with the model group （P 〈 0.05, P 〈 0.01 ）. There was no significant difference between the two treatment groups （ P 〉 0.05 ）. NO level was significantly decreased and ET- 1 level was increased in model rats （P 〈 0.01 or P 〈 0.05 ）. Huangqi Weimao mixture treatment can signi{lcantly in- crease NO level and reduce ET - 1 level （ P 〈 0.05 ）. There was no significant difference compared with model group and ]osartan potassium group （ P 〉 0.05 ）. Conclusion ： Huangqi Weimao mixture can increase VEGF expression and reduce TSP - 1 expression in renal glomerulus and can increase the content of NO and reduce ET - 1 level, regulate VEGF, TSP - 1 and NO, ET - 1 bal- ance. Therefore it can protect glomerular endothelial cells, promote blood capillary and small vessel regeneration an

关 键 词：糖尿病肾病 链脲佐菌素 血管内皮细胞生长因子 血小板结合蛋白-1 

分 类 号：R587.2[医药卫生—内分泌]