辣椒组织培养再生体系的影响因素研究  

Study on Influencing Factors for Regeneration System of Pepper Tissue Culture

作　　者：曲静[1] 刘思言[2] 姚丹[2] 关淑艳[2] 刘志刚 蔡力南 王丕武[1] 

机构地区：[1]吉林农业大学农学院,吉林长春130118 [2]吉林农业大学生命科学学院,吉林长春130118 [3]吉林省育华药业有限责任公司,吉林长春130118

出　　处：《山东农业科学》2014年第6期27-29,34,共4页Shandong Agricultural Sciences

基　　金：吉林农业大学科研启动基金(201242);吉林省科技厅科技发展计划项目(201101111);吉林省教育厅科学技术研究项目(2011-41);吉林省科技厅项目(20120215);吉林省科技厅成果转化补助项目(20095044)

摘　　要：以早熟辣椒、红线辣椒、茄门大辣椒(绿辣椒)3个品种为材料,研究了辣椒组织培养再生体系的影响因素。结果表明:红线辣椒为最佳基因型,辣椒离体再生最适外植体为茎段、子叶。诱导茎段愈伤生成的最佳培养基为MS+0.10 mg/L 6-BA+2.0 mg/L NAA;子叶愈伤生成的最佳培养基为MS+0.10 mg/L6-BA+4.0 mg/L NAA;高效不定芽分化培养基为MS+5.0 mg/L 6-BA+0.1 mg/L IAA;高效不定根分化培养基为MS+0.5 mg/L IBA+0.1 mg/L NAA。Three varieties including premature pepper , Red pepper and Qiemen pepper （ Green pepper ） were used as materials to study the factors influencing regeneration system of pepper tissue culture .The results showed that the genotype of Red pepper was optimum .Stem segment and cotyledon were the optimum explants for in vitro regeneration, and the medium for their callus induction were MS ＋0.10 mg/L 6-BA＋2.0 mg/L NAA and MS＋0 .10 mg/L 6-BA＋4 .0 mg/L NAA respectively .The medium for high-efficient differentia-tion of adventitious buds was MS ＋5.0 mg/L 6-BA＋0.1 mg/L IAA, and that for differentiation of adventi-tious roots was MS＋0.5 mg/L IBA ＋0.1 mg/L NAA.

关 键 词：辣椒 愈伤组织 植株再生 

分 类 号：S641.303[农业科学—蔬菜学]