比较小檗碱对HEPG2蛋白质组的抗肿瘤作用  

Comparative proteomic study on antitumor effect of berberine on human hepatocellular carcinoma cell line HEPG2

作　　者：陈华[1] 谷容[1] 赵晓东[1] 刘雯[1] 袁拥华[1] 

机构地区：[1]重庆医科大学附属儿童医院药学部,重庆400014

出　　处：《重庆医学》2014年第18期2303-2304,2311,共3页Chongqing medicine

基　　金：重庆市卫生局中医处课题(2009-2-47)

摘　　要：目的探讨小檗碱对人肝癌细胞株HEPG2蛋白表达的影响及其抗肿瘤作用的分子机制。方法采用蛋白质组学技术比较小檗碱处理人肝癌细胞株HEPG2前、后蛋白表达的变化。结果筛选出差异表达蛋白共38个,16个蛋白表达上调,22个蛋白表达下调,质谱鉴定出10个蛋白点。结论小檗碱的抗肿瘤效应可能与过氧化物酶增殖物激活受体γ(PPARγ)信号转导通路有关,PPARγ可能是小檗碱的作用靶点。Objective To research the effects of berberine on the protein expression of human hepatocellular carcinoma cell line HEPG2 for elucidating its antitumor mechanism.Methods The change of protein expression of human hepatocellular carcinoma cell line HEPG2 induced by berberine were analyzed by using comparative proteomics.Results A total of 38 differentially expressed protein spots were detected.16 protein spots were up-regulated and 22 protein spots were down-regulated.10 protein spots were successfully identified by the mass spectrum.Conclusion The berberine antitumor effect may be related with PPARγsignal trans-duction pathway,PPARγmay be the target of berberine.

关 键 词：小檗碱 蛋白质组学 抗肿瘤 

分 类 号：R285[医药卫生—中药学]