电刺激迷走神经对高脂饮食大鼠摄食和体重的影响  

The Study of Electrical Vacus Nerve Stimulation on Food Consumption And Weight Gain in Rats Feded the High-fat Diet

作　　者：李阁[1] 王巧玲[1] 逄明杰[2] 祝海[2] 徐珞[1] 

机构地区：[1]青岛大学医学院病理生理学教研室,山东青岛266021 [2]青岛市立医院

出　　处：《菏泽医学专科学校学报》2014年第2期1-5,19,共6页Journal of Heze Medical College

基　　金：青岛市科技局项目(13-1-4-170-jch)

摘　　要：目的观察长期高频(10Hz)电刺激迷走神经(VNS)对高脂饮食大鼠摄食、体重及激素分泌的影响,探讨迷走神经调控摄食和体重的作用机制。方法成年雄性Wistar大鼠皮下植入微电极(MS),电极末端包裹膈下迷走神经,每日18:00时到第天6:00时刺激大鼠左侧迷走神经(方波:10 ms,200 mV,10 Hz),整个实验过程中均给予大鼠高脂饮食(42天);每天早晨测量体重和摄食量;实验末大鼠断头取血,用放射免疫分析法检测血清ghrelin、瘦素和nesfatin-1水平;称量附睾脂肪用来评估脂肪组织含量。采用免疫组化检测孤束核(NTS)c-Fos神经元表达。结果与对照组及假手术组相比,长期电刺激迷走神经,可显著减少大鼠摄食量(P<0.05),抑制体重增加(P<0.05),并降低附睾脂肪重量(P<0.05);长期电刺激迷走神经,血清Ghrelin水平显著升高(P<0.05),但瘦素水平明显降低(P<0.05),nesfatin-1水平升高不明显(P>0.05);刺激左侧迷走神经,两侧NTS c-Fos活性细胞数量均显著增加,左侧增加更明显(P<0.05)。结论迷走传入可增强脑饱感信号传导,从而减少摄食、体重及脂肪含量。VNS可诱发摄食相关激素反应。Objective To investigate the effect of high frequency （10 Hz） VNS on feeding behavior, appetite, peptide secretion in rats fed a high-fat diet, and to explore the mechanisms of VNS on the regulation of feeding and weight. Methods Adult male Wistar rats were done laparotomy surgery, each implanted subcutaneously with a microstimulator （MS）. The vagus nerves beneath diaphragma were wrapped by MS. The left vagus nerve was stimulated by rectangular electrical pulses （10 ms, 200 mV, 10 Hz, 12 h a day） generated by the MS. Body weight and food intake were measured each morning. At the end of the experimental period, animals were euthanized and blood samples were taken. Serum levels of ghrelin, leptin and nesfatin-1 were assessed using radioimmunoassays. Adipose tissue content was evaluated by weighing epididymal fat pads, which were incised at the time of sacrifice. Using immunohistochemical to detecte the expression of c-Fos neurons in solitary nucleus （NTS）. Results Chronic vagus nerve stimulation significantly decreased food intake（P〈0.05）, body weight gain （P〈0.05） and epididymal fat pad weight （P〈0.05） in animals that received VNS compared with control animals. Signifi- cant neuronal responses in the NTS were observed following VNS. Finally, compared with control animals and sham group, chronic vagus nerve stimulation group serum concentrations of ghrelin were increased（P〈0.05）, while serum levels of leptin were decreased（P〈0.05）. Although not significant, serum nesfatin-1 levels were also elevated. Conclusions VNS leads to reductions in food intake, body weight gain and adipose tissue by increasing brain satiety signals conducted through the vagal afferents. VNS also evoked a feed-related hormonal response.

关 键 词：体重 GHRELIN 瘦素 NESFATIN-1 孤束核 迷走神经刺激 

分 类 号：R542.22[医药卫生—心血管疾病]