HLA系统D区基因分型和中国汉族人特有的RFLP  

The Genotyping of HLA-D Region and Specific RFLP Patterns for Chinese Han population

作　　者：费虹明[1] 蒋伟宏[1] 陈仁彪[1] 范丽安[2] 徐可愚[3] 

机构地区：[1]上海第二医科大学生物学教研室,200025 [2]上海市免疫学研究所免疫遗传研究室 [3]中国医学科学院皮肤病研究所

出　　处：《Acta Genetica Sinica》1993年第2期102-110,共9页

摘　　要：HLA Ⅱ类抗原在外来抗原提呈中起重要作用。在DNA水平进行Ⅱ类抗原基因分型有更强的多态性鉴别能力,有利于检出新的特异性。根据白种人纯合分型细胞(HTC)和中国人部分纯合细胞DRB和DQA基因的RFLP格局,我们对上海地区90名随机健康人进行了HLA-D区基因分型,并将结果与血清学分型相比较,证明了它的可靠性和优越性。此外,通过与白种人格局相比,我们还发现了中国人特有的HLA-D区RFLP格局。The HLA class Ⅱ antigens play very an important role in presenting foreign antigens. The class Ⅱ gene typing at the DNA level possesses higher capability to recognize polymorphism so that it can disclose new specificities. According to the RFLP patterns of DRB and DQA genes for Caucasian HTC and some Chinese homozygous cells, we typed 90 randomly sampled healthy Chinese Hans in Shanghai area, and compared the results obtanied with those from serological typing. In addition to being reliable and superior to serological typing, we found some unique RFLP patterns of the HLA-D region in Chinese Han population based on comparison with Caucasian patterns.

关 键 词：基因分型 印迹法 RFLP 汉族 

分 类 号：Q987[生物学—遗传学]