龙牡桂枝汤改善注意缺陷多动障碍模型大鼠行为的实验研究  

Behavioral study of Longmu Guizhi decoction on ADHD model rats

作　　者：何平[1] 唐彦[2] 杨若俊[2] 

机构地区：[1]云南中医学院第一附属医院儿科,昆明650021 [2]云南中医学院儿科教研室,昆明650021

出　　处：《中国中西医结合儿科学》2014年第3期203-205,共3页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：云南省科技厅自然科学基金面上项目(2010CD074)

摘　　要：目的观察龙牡桂枝汤对注意缺陷多动障碍(ADHD)模型动物——幼年自发性高血压大鼠(SHR)的自发活动和非选择性注意力的影响。方法采用幼年雄性SHR大鼠48只,随机分为龙牡桂枝汤高、中剂量组、盐酸哌甲酯组和生理盐水组,连续灌胃给药14d。通过开场实验和Làt迷宫实验分别检测给药前后大鼠自发活动和非选择性注意力的变化。结果在开场实验中,各药物组穿越格子数和直立次数明显少于生理盐水组,差异有统计学意义(P<0.01);盐酸哌甲酯组大鼠穿越格子数和直立次数少于中药中剂量组,差异有统计学意义(P<0.05),而中药高剂量组与盐酸哌甲酯组比较差异无统计学意义(P>0.05)。Làt迷宫实验中,各药物组斜搭次数多于生理盐水组,差异有统计学意义(P<0.01),中药高剂量组的斜搭次数多于中剂量组及盐酸哌甲酯组,差异有统计学意义(P<0.05)。结论龙牡桂枝汤可改善ADHD模型大鼠的自发活动和非选择性注意力,并呈现出一定的量效关系。Objective To investigate the effect of Longmu Guizhi decoction（LD）on locomotor activity and non-selective attention of juvenile SHR（spontaneously hypertensive rat）——a rat model of attention deficit hyperactivity disorder.Methods Forty-eight SHR male were randomly divided into high-dose LD group,middle-dose LD group,methylphenidate control group and saline control group.All the SHRs were lavaged for 14 days.Locomotor activity and non-selective attention were examined by open-field test and Làt mazetest respectively before and after intervention.Results In open-field test,the number of square crossing and rearing after intervention of methylphenidate and decoctions in SHRs was significantly fewer than the saline group（P〈0.01）.The number of the above items in methylphenidate group was significantly fewer than middle-dose LD group（P〈0.05）.There was no difference between methylphenidate group and high-dose LD group（P〉0.05）.In Làt maze test,frequency of leaning against the wall after intervention of methylphenidate and decoctions in SHRs was significantly increased compared with the saline group（P〈0.01）,and that of high-dose LD group was significantly higher than the methylphenidate group and middle-dose LD group（P〈0.05）.Conclusion Longmu Guizhi decoction can reduce locomotor activity and improve non-selective attention of ADHD model rats,which presents some kind of dose-effect relationship.

关 键 词：轻微脑损伤综合征 龙牡桂枝汤 动物 实验 大鼠 

分 类 号：R33[医药卫生—人体生理学]