常用人工抗原鉴定方法的比较  

Study of Comparison the Commonly Used Artificial Antigen Identification Methods

作　　者：郭乃菲[1] 于基成[2] 马福敏[1] 郭小瑞[1] 徐美玲[1] 党亚丽[3] 范永梅[1] 宫凯莹 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]大连民族学院生命科学学院,辽宁大连116600 [3]浙江省医学科学院保健食品所,浙江杭州310013

出　　处：《食品与生物技术学报》2014年第5期517-521,共5页Journal of Food Science and Biotechnology

基　　金：浙江省卫生厅科技项目(2011KYB002)

摘　　要：以杀菌剂百菌清为基础制备人工抗原,分别使用Native-PAGE电泳法、SDS-PAGE电泳法和紫外分光光度法对其进行鉴定,并测定各人工抗原的偶联比。研究结果表明,使用紫外分光光度法鉴定人工抗原时,存在假阳性情况,此时仍可计算出人工抗原的偶联比。若使用假阳性人工抗原进行免疫,所获得的抗体效价较低,对载体蛋白的识别能力较强,不适合用于免疫检测技术的建立。Research purpose of the paper was comparing the commonly identification methods of Artificial antigen,such as Native-PAGE electrophoresis,SDS-PAGE electrophoresis and UV spectro-photometry.In the paper,as the fungicide chlorothalonil was the based compound to preparate the artificial antigens.Native-PAGE electrophoresis,SDS-PAGE electrophoresis and UV spectro-photometry were used of qualitative analysising the artificial antigens,respectively.The results showed that the UV spectrophotometry identification method might cause the false positive,but it still could calculate the coupling ratio of the artificial antigen.If the false positive artificial antigen was used as immunizing antigen,the titer of antibody be low,while,the recognition ability with the carrier protein be strength,which is not suitable for establishing the immunoassay technology.

关 键 词：人工抗原 紫外扫描 非变性凝胶电泳 聚丙烯酰胺凝胶电泳 偶联比 

分 类 号：R392[医药卫生—免疫学]