渗透胁迫对山黧豆幼苗H_2O_2及毒素积累的影响  

Effects of Osmotic Stress on Accumulation of H_2O_2 and ODAP in Grass Pea ( Lathyrus sativus )

作　　者：邢更生[1] 周功克[1] 李志孝[2] 崔凯荣[1] 

机构地区：[1]兰州大学干旱农业生态国家重点实验室,兰州730000 [2]兰州大学应用有机化学国家重点实验室,兰州730000

出　　处：《植物生理学报（0257-4829)》2001年第1期5-8,共4页Acta Phytophysiologica Sinica

基　　金：国家自然科学基金资助项目 !(批准号 39770 46 9)

摘　　要：用PEG经根部处理 15d龄的山黧豆幼苗 ,取幼苗叶片为实验材料 ,测定过氧化氢酶 (CAT)、过氧化物酶 (POD)活性、过氧化氢 (H2 O2 )和毒素 ( β N oxalyl α ,β diaminopropionicacid ,ODAP)的含量。结果表明 ,随着PEG处理时间的延长 ,POD和CAT活性降低 ,而H2 O2 和ODAP含量显著升高 ;在PEG处理液中加入二乙基二硫代氨基甲酸钠 (diethyldithiocarbamate ,DDC)和氨基三唑 (aminotriazole ,AT)后分别抑制和促进H2 O2 的产生 ,DDC可降低叶片中ODAP的含量 ,AT则使ODAP积累。由此我们推测 。Roots of fifteen day old grass pea seedlings were treated with PEG and then the leaves were used as material. Changes in activities of catalase and peroxidase, and hydrogen peroxide and β N oxalyl α, β diaminopropionic acid (ODAP) contents during PEG treatment, were assayed. The results indicated that during PEG treatment, activities of peroxidase and catalase decreased (Fig.3), and hydrogen peroxide (Fig.2) and ODAP content (Fig.1) increased greatly. Diethyldithiocarbamate or aminotriazole strongly decreased or increased respectively the H 2O 2 content (Fig.2). While the accumulation of ODAP was decreased markedly by diethyldithiocarbamate, it was increased significantly by aminotriazole (Fig.1). Therefore, we suggest that the accumulation of ODAP might be related to the increase in H 2O 2 content under osmotic stress.

关 键 词：渗透胁迫 山黧豆 过氧化氢 毒素 抗氧化酶 豆科 

分 类 号：Q945.78[生物学—植物学] Q949.751.9