流动剪切力对鼠脑微血管内皮细胞ICAM-1表达的影响  

ICAM-1 expression of rat brain microvascular endothelial cells caused by fluid shear force

作　　者：宋晓燕[1] 曾衍钧[1] 李彩霞[1] 廖东华[1] 胡金麟[2] 郝延磊[3] 

机构地区：[1]北京工业大学生物力学和医学信息研究所,北京100022 [2]中国人民解放军总医院微循环室,北京100853 [3]中国人民解放军总医院神经内科实验室,北京100853

出　　处：《生理学报》2001年第1期13-17,共5页Acta Physiologica Sinica

基　　金：ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (No 396 0 0 0 40 )andBeijingNaturalScienceFoundation (No 3982 0

摘　　要：利用内皮细胞流动小室方法 ,对大鼠脑微血管内皮细胞在剪切力作用下细胞内粘附分子 1(ICAM 1,intercellularadhesionmolecule 1)的表达进行了研究。图像分析结果提示 ,脑微血管内皮细胞在剪切力作用下ICAM 1的表达呈特异上调 ,且存在着时间依赖性 ,与一定范围内的剪切力强度无关。用对细胞施加剪切力作用后提取上清液孵育内皮细胞的方法证明 :剪切力对鼠脑微血管内皮细胞ICAM 1表达的影响 ,是直接作用于内皮细胞引起的细胞内的直接反应 ,而不是剪切力导致细胞先释放细胞因子 ,释放的细胞因子再引起ICAM 1变化的间接反应。We studied the effect of shear stress (SS) on intercellular adhesion molecule 1 (ICAM 1) expression of rat brain microvascular endothelial cells (RBMECs) using the parallel plate flow chamber method. It was demonstrated that RBMECs showed a time dependent, but not a force dependent, upregulation in ICAM 1 expression. Endothelial cell surface expression of ICAM 1 in the supernatants of RBMECs exposed to SS was not changed, thus excluding the possibility that the upregulated expression is due to the factors synthesized by these cells. These data throw light on understanding the signal transduction pathway inside the endothelial cells under the effect of SS.

关 键 词：剪切力 鼠脑微血管内皮细胞 内皮细胞粘附分子 

分 类 号：R331.1[医药卫生—人体生理学]