HCVE2区基因的分子克隆及序列分析  

Molecular Cloning and Sequencing of HCV E2 Gene

作　　者：张东伟[1] 梁布锋[1] 祁自柏[2] 凌世淦[3] 

机构地区：[1]中国科学院武汉病毒研究所,湖北武汉430071 [2]中国药品生物制品检定所,北京100050 [3]军事医学科学院,北京100850

出　　处：《中国病毒学》2001年第1期40-44,共5页Virologica Sinica

基　　金：国家九五科技攻关基金资助项目 !( 96 90 6 0 3 0 6 )

摘　　要：用RT PCRKIT从西安血站大样抗HCV阳性血清中筛选出HCVRNA阳性血清 ,提取HCV的RNA ,利用随机引物反转录合成其cDNA并进行半巢式PCR反应。将纯化的PCR产物酶切后与表达载体PET 2 2b+连接 ,经过双脱氧末端终止法双向测序 ,得到 85 2bp长的核苷酸序列。通过将该序列与已知不同型的HCVE2序列比较得知 。HCV RNA positive serum was first selected by RT PCR test kit from several anti HCV positive sera obtained from Xi'an.HCV RNA extracted from the elected sera was converted to cDNA by reverse transcription with random primer.Half nested PCR was performed.The amplified product was 852 bp.The purified PCR product was digested by restriction endonucleases and then ligated to epressio vector pET 22b\++.Its nucleotide sequence was determined by dideoxy chain termination method.A comparison of the sequence with several isolates reported previously showed that the sequence belonged to HCV type Ⅱ.

关 键 词：丙型肝炎病毒 E2包膜糖蛋白 HVR1 序列特征 

分 类 号：Q785[生物学—分子生物学]