女贞子连续超声逆流提取工艺优选  

Optimization of Successive Ultrasound Countercurrent Extraction Technology of Ligustri Lucidi Fructus

作　　者：张静[1] 陈红专[1] 董立彬[1] 王海亮[1] 

机构地区：[1]石家庄以岭药业股份有限公司,石家庄050035

出　　处：《中国实验方剂学杂志》2014年第13期16-18,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2011ZX09401-306)

摘　　要：目的:优选女贞子的连续超声逆流提取工艺。方法:以齐墩果酸和熊果酸总量为指标,通过正交试验考察超声频率、乙醇体积分数、料液比和温度对提取工艺的影响。采用HPLC测定齐墩果酸和熊果酸含量,流速0.33 mL·min-1,流动相乙腈-甲醇-0.5%冰醋酸溶液(19∶67∶14)。结果:最佳提取工艺为加15倍量80%乙醇于30 kHz提取;齐墩果酸和熊果酸平均总质量分数5.66%,RSD 1.64%。结论:优选的连续超声逆流提取工艺操作简单、科学合理,适合于女贞子工业化大生产。Objective： To optimize successive ultrasound countercurrent extraction process of Ligustri Lucidi Fructus. Method： Taking total amount of oleanolic acid and ursolic acid as index, orthogonal design was adopted to investigate effects of ethanol concentration, ultrasonic frequency, solid-liquid ratio and temperature on extraction process of Ligustri Lueidi Fructus. ELSD was employed to determine contents of oleanolic acid and ursolic acid with mobile phase of acetonitrile-methanol-0.5% acetic acid （ 19 ： 67 ： 14 ） and flow rate of 0.33 mL ·min^-1 Result： Optimum extraction process was that extracted with 15 times the amount of 80% ethanol at 30 kHz; under these conditions, average total amount of oleanolic acid and ursolic acid was 5.66% with RSD of 1.64%. Conclusion： This optimized extraction technology was reasonable and scientific, which was suitable for large scale production of Ligustri Lucidi Fructus.

关 键 词：女贞子 连续超声逆流法 齐墩果酸 熊果酸 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]