中药乳腺康注射液中间体的制备工艺优选  

Optimization of Preparation Technology for Intermediates of Ruxiankang Injections

作　　者：刘涛[1] 游丽玲[1] 刘应武[1] 刘婷[1] 李娟 伍利华[3] 徐玉玲[4] 

机构地区：[1]成都大学生物产业学院,成都610106 [2]南京海陵中药制药工艺技术研究有限公司,南京210049 [3]广西中医药大学,南宁530001 [4]成都大学实验技术中心,成都610106

出　　处：《中国实验方剂学杂志》2014年第13期22-25,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省卫生厅科研项目(130405)

摘　　要：目的：优选中药乳腺康注射液中间体的制备工艺.方法：以总含固量和橙皮苷含量的综合评分为指标,通过正交试验和单因素试验考察乙醇用量、提取时间、提取次数及乙醇体积分数对甘草和青皮的混合提取工艺的影响;以总黄酮质量和总含固量为综合评价指标,采用正交试验考察加水量、提取时间及提取次数对蒲公英提取工艺的影响,通过单因素试验考察含醇量对蒲公英提取液醇沉工艺的影响.采用HPLC测定橙皮苷含量,流动相甲醇-水（50∶50）,检测波长284 nm.结果：甘草和青皮的最佳提取工艺为加6倍量60％乙醇提取3次,每次2h.蒲公英最佳提取工艺为加16倍量水浸泡0.5h后提取3次,每次0.5h;提取液减压浓缩至相对密度1.15 ～1.25（60 ～ 80℃）,85％乙醇沉淀,回收乙醇至相对密度1.25（60～80℃）.结论：优选的提取、纯化工艺稳定可行,为中药乳腺康注射液的制备提供参考.Objective： To optimize preparation technology of intermediates of Ruxiankang injections. Method： With composite score of contents of total solids and hesperidin as index, orthogonal test and single factor test were adopted to investigate effects of extracting time, extraction times, ethanol concentration and amount on technology of Glycyrrhizae Radix et Rhizoma and Citri Reticulatae Pericarpium Viride. Taking contents of total flavonoids and solids as comprehensive evaluation index, effects of the amount of water, extracting time and times on extraction process of Taraxaci Herba was investigated by orthogonal test, single factor test was adopted to optimize alcohol precipitation process. HPLC was employed to determine the content of hesperidin with mobile phase of methanol-water （50： 50） and detection wavelength at 284 nm. Result： Optimum extraction technology of Glycyrrhizae Radix et Rhizoma and Cirri Reticulatae Pericarpium Viride was as following： extracted 3 times with 6 times the amount of 60% ethanol for 2 h each time. Optimum extraction process of Taraxaci Herba was adding 16 times the amount of water for soaking 0.5 h, extracted 3 times with 0.5 h for each time. Extract was concentrated under vacuum to a relative density of 1.15-1.25 （60-80 ℃ ） , added 95% ethanol slowly to ethanol amount of 85% , recovered ethanol to a relative density of 1.25 （60-80 ℃ ）. Conclusion： These optimized processes were stable and feasible, it could provide a reference for preparation of Ruxiankang injections.

关 键 词：乳腺康注射液 橙皮苷 总黄酮 正交试验 总含固量 醇沉工艺 甘草 蒲公英 青皮 

分 类 号：R283.6[医药卫生—中药学] R944.9[医药卫生—中医学]