小儿七星茶颗粒指纹图谱  

Fingerprint of Xiaoerqixingcha Granules

作　　者：朱焕容[1,2] 赵小宁[3] 王秀芬[2] 曾惠芳[3] 苏子仁[2] 彭绍忠 黄晓丹 郑荣波 

机构地区：[1]广州王老吉药业股份有限公司,广州510450 [2]广州中医药大学中药学院,广州510006 [3]广州中医药大学第一附属医院,广州510405

出　　处：《中国实验方剂学杂志》2014年第13期93-96,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立广州王老吉药业股份有限公司三公仔牌小儿七星茶颗粒HPLC指纹图谱分析方法,科学评价并有效控制其颗粒剂的质量,保证其生产的稳定性。方法:采用反相高效液相色谱法,SymmetryC18色谱柱(4.6 mm×250 mm,5μm),乙腈-0.2%乙酸溶液梯度洗脱,柱温30℃,流速1.0 mL·min-1,检测波长254 nm。结果:以甘草苷为参照峰,建立了三公仔牌小儿七星茶颗粒HPLC指纹图谱,确立了20个共有峰,10批样品之间的共有峰相似度>0.9。结论:该方法准确,精密度、重复性较好,可作为控制广州王老吉药业股份有限公司三公仔牌小儿七星茶颗粒质量的依据。Objective： To establish a sensitive HPLC fingerprint analytical method for evaluating scientifically and controlling the quality of Xiaoerqixingcha granule manufactured by Guangzhou wanglaoji Pharmaceutical Company Limited. Method： RP-HPLC analysis was performed at 30 ℃ by using a Symmetry C18 column （4.6 mm × 250 mm, 5 μm）, with the gradient elute of mobile phase consisting of acetonitrile-0.2% glacial acetic acid at the flow rate of 1.0 mL·min^-1. The UV detection was set at 254 nm. Result： Liquiritin was used as the reference substance in the HPLC chromatographic fingerprint of Xiaoerqixingcha Granule, which showed 20 common peaks and the similarity was more than 0.90 among 10 samples. Conclusion： This method was accurate, repeatable and useful for the quality control of Xiaoerqixingcha granules of Guangzhou wanglaoji Pharmaceutical Company Limited.

关 键 词：小儿七星茶颗粒 甘草苷 绿原酸 芹菜素 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]