基于主成分分析和硫熏前后无机元素含量变化的硫熏与非硫熏菊花鉴别方法  

Identification Method for Sulphur Fumigated Chrysanthemi Flos based on Principal Component Analysis and Element Contents

作　　者：汤坚[1] 汪华君[1] 蒋俊[2,3,4] 顾丽亚[3,4] 李昊天[3,4] 李平[2] 贾晓斌[3,4] 李松林[4] 

机构地区：[1]江苏大学附属医院药剂科,江苏镇江212001 [2]镇江出入境检验检疫局,国家食品添加剂及调味品检测重点实验室,江苏镇江212008 [3]南京中医药大学药学院,南京210023 [4]江苏省中医药研究院,国家中医药管理局中药释药系统重点研究室,南京210028

出　　处：《中国实验方剂学杂志》2014年第13期97-101,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省镇江市科技支撑计划-社会发展项目(SH2012010);江苏大学医学临床科技发展基金项目资助(JLY20120170)

摘　　要：目的:研究菊花硫磺熏蒸前后样品中多种无机元素含量差异性,并探索根据无机元素含量变化,结合主成分分析建立硫熏菊花的定性区分识别方法。方法:采用Ultrwave digestion/ICP-MS检测硫磺熏蒸前后菊花中多种无机元素含量,结合主成分分析(PCA)散点分布法对硫熏和非硫熏菊花进行区域划分,建立其区分鉴别的方法;并随机抽取9批样品,考察已建立方法的实用性。结果:比较9批硫熏菊花样品与9批非硫熏菊花样品的无机元素含量,Ba和Cu元素含量在硫磺熏蒸后含量增加且具有显著性;Pb和As元素含量略有增加但不具有显著性;Hg元素含量无明显变化;Cd元素含量较非硫熏菊花样品略有降低但不具有显著性;PCA分析9批硫熏菊花和9批非硫熏菊花的区域分布相对较散,差异较大,能够完成硫熏菊花和非硫熏菊花的区分鉴定;此外,该方法还能对未知菊花样品进行初步定性分析。结论:该方法符合测定要求,可应用于硫熏菊花的质量控制及安全性考察,为探索硫熏菊花和其他硫熏中药的区分鉴别提供了重要的数据。Objective： To Study Chrysanthemi Flos variety of elements content in the samples before and after sulphur fumigated differences, and try to establish sulphur fumigated Chrysanthemi Flos qualitative identification method. Method： Using Ultrwave Digestion/ICP-MS testing before and after sulphur fumigated Chrysanthemi Flos, a variety of mental elements content in combined with principal component analysis （PCA） scatter distribution method to distinguish between sulphur fumigated and non sulphur fumigated Chrysanthemi Flos. Result： Nine batches of storing of sulphur fumigated Chrysanthemi Flos samples and nine samples of each element content comparison found that Ba and Cu element content increased after sulphur fumigated Chrysanthemi Flos medicine materials and has significant; Pb and As element content increased slightly, but not significant; Hg element content has no obvious change; Cd element content in the sulphur fumigated Chrysanthemi Flos samples slightly lower but not significant; 9 batches of sulphur fumigated Chrysanthemi Flos and 9 batches non-sulphur fumigated Chrysanthemi Flos distribution distance is relatively loose, able to complete stoved Chrysanthemi Flos and non-sulphur fumigated Chrysanthemi Flos to distinguish the identification. Conclusion： Methodology to investigate the results show that this method conform to the requirements of the measurement, the method can be applied to quality control of sulphur fumigated Chrysanthemi Flos and safety inspection, to explore stored Chrysanthemi Flos and other sulphur fumigated the distinction between tradition Chinese medicine identification provides important data.

关 键 词：硫磺熏蒸 菊花 Ultrwave DIGESTION ICP-MS 主成分分析 无机元素 鉴别 

分 类 号：R284.1[医药卫生—中药学]